Abstract
Different seed preconditioning treatments and light intensities were tested for their effect on the callus induction and regeneration in cotyledon cultures of mungbean ( Vigna radiata (L.) Wilczek). There were six preconditioning treatments evaluated including imbibition in sterile distilled water, inoculation in plain agar, and inoculation in 6-benzyladenine (6-BA) supplemented media over a varing period of time. The cultures were incubated in two light intensity conditions; namely, full light (50 μmol m-2 sec-1) and minimum light (8.5 μmol m-2 sec-1). Results of the study showed that (1) plain imbibition of seeds in sterile distilled water for 16 hours was more favorable for regeneration than preconditioning with 6-BA enriched media, and (2) preconditioning of callus formation and better callus growth. Other important observation. was the differential response to plantlet regeneration under different regeneration media. Plantlet regeneration in B5 basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BA was relatively higher than the other regeneration media under full light intensity while Murashigc and Skoog (MS) basal medium and MS basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BA had highter plantlet regeneration under minimum light intensity.
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Claims 4
Claim · model-written summary
Imbibition in sterile water for 16 hours favored regeneration over 6-BA medium preconditioning.
- Taxon
- mungbean
.jpg?width=60)
Vigna radiatae1 - Treatment
- plain imbibition of seeds in sterile distilled water for 16 hourse0
- Outcome
- regeneratione0
- Direction
- more favorablee0
- Comparator
- preconditioning with 6-BA enriched mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“plain imbibition of seeds in sterile distilled water for 16 hours was more favorable for regeneration than preconditioning with 6-BA enriched media”
e1“mungbean ( Vigna radiata (L.) Wilczek)”
Claim · model-written summary
Under full light, B5 medium with NAA and 6-BA gave relatively higher plantlet regeneration than other media.
- Taxon
- mungbeanVigna radiatae1
- Treatment
- B5 basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BAe0
- Outcome
- Plantlet regeneratione0
- Direction
- relatively highere0
- Qualifier
- full light intensitye0
- Comparator
- other regeneration mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plantlet regeneration in B5 basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BA was relatively higher than the other regeneration media under full light intensity”
e1“mungbean ( Vigna radiata (L.) Wilczek)”
Claim · model-written summary
Under minimum light, MS basal medium had higher plantlet regeneration.
- Taxon
- mungbeanVigna radiatae1
- Treatment
- Murashigc and Skoog (MS) basal mediume0
- Outcome
- plantlet regeneratione0
- Direction
- hightere0
- Qualifier
- minimum light intensitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Murashigc and Skoog (MS) basal medium and MS basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BA had highter plantlet regeneration under minimum light intensity”
e1“mungbean ( Vigna radiata (L.) Wilczek)”
Claim · model-written summary
Under minimum light, supplemented MS medium had higher plantlet regeneration.
- Taxon
- mungbeanVigna radiatae1
- Treatment
- MS basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BAe0
- Outcome
- plantlet regeneratione0
- Direction
- hightere0
- Qualifier
- minimum light intensitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BA had highter plantlet regeneration under minimum light intensity”
e1“mungbean ( Vigna radiata (L.) Wilczek)”
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