Abstract
Abstract A monoclonal antibody, designated YMV‐M24, against yam mosaic virus (YMV), genus Potyvirus , was used to develop a triple antibody sandwich (TAS)‐ELISA, dot blot immunoassay (DBI), direct tissue blotting immunoassay (DTBI) and an immunosorbent electron microscopy (ISEM) for the detection of the virus in yam tissues. DBI was most sensitive. DTBI detected YMV in yam tubers and tuber sprouts. TAS‐ELISA, DBI and DTBI showed that the concentration of YMV increased from the base towards the tip of yam tuber sprouts. YMV‐M24 decorated YMV particles in ISEM. Copyright © 2004 Whurr Publishers Ltd
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Claims 4
Claim · model-written summary
DBI was the most sensitive detection assay.
Verbatim
e0“DBI was most sensitive.”
t0“yam mosaic virus in yam tissues”
Claim · model-written summary
DTBI detected YMV in yam tubers and sprouts.
Verbatim
e0“DTBI detected YMV in yam tubers and tuber sprouts.”
Claim · model-written summary
Three assays showed YMV concentration increasing from sprout base to tip.
Verbatim
e0“TAS‐ELISA, DBI and DTBI showed that the concentration of YMV increased from the base towards the tip of yam tuber sprouts.”
Claim · model-written summary
YMV-M24 decorated virus particles in ISEM.
Verbatim
e0“YMV‐M24 decorated YMV particles in ISEM.”
t0“yam mosaic virus in yam tissues”