Abstract
Taxus baccata L. roots collected from two sites in southern Germany were heavily colonized by arbuscular mycorrhizal fungi (AMF). The colonization pattern was of the Paris type. The diversity of the colonizing AMF species was investigated using polymerase chain reaction based molecular techniques. The internal transcribed spacer (ITS) region of the DNA from AMF within the roots was amplified using Glomeromycota-specific primers and then cloned and sequenced. Phylogenetic analysis using a data set of 5.8S rDNA sequences from a wide range of glomeralean taxa as well as data sets of partial ITS2 sequences from glomeralean subgroups indicated root colonization by four sequence types of Glomus and one sequence type of Archaeospora. These sequence types are distinct from any previously published sequences and differed between the two study sites.Key words: arbuscular mycorrhiza, Paris-type AM, molecular diversity, ribosomal internal transcribed spacers, Taxus baccata.
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Claims 6
Claim · model-written summary
Taxus baccata roots at two southern German sites were heavily colonized by AMF.
- Taxon
- Taxus baccata
Taxus baccatae0 - Interactor
- arbuscular mycorrhizal fungie0symbiont
- Outcome
- heavily colonizede0
- Setting
- southern Germanye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Taxus baccata L. roots collected from two sites in southern Germany were heavily colonized by arbuscular mycorrhizal fungi (AMF).”
Claim · model-written summary
The colonization pattern was Paris type.
- Taxon
- Taxus baccataTaxus baccatat0
- Outcome
- colonization pattern was of the Paris typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The colonization pattern was of the Paris type.”
t0“Taxus baccata”
Claim · model-written summary
Roots contained four Glomus sequence types.
- Taxon
- Taxus baccataTaxus baccatat0
- Outcome
- four sequence types of Glomuse0
- Value
- four sequence typese0
GlomusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root colonization by four sequence types of Glomus and one sequence type of Archaeospora”
t0“Taxus baccata”
Claim · model-written summary
Roots contained one Archaeospora sequence type.
- Taxon
- Taxus baccataTaxus baccatat0
- Interactor
- ArchaeosporaArchaeosporae0symbiont
- Outcome
- one sequence type of Archaeosporae0
- Value
- one sequence typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one sequence type of Archaeospora”
t0“Taxus baccata”
Claim · model-written summary
The AMF sequence types differed from previously published sequences.
- Taxon
- Taxus baccataTaxus baccatat0
- Outcome
- sequence types are distincte0
- Comparator
- previously published sequencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These sequence types are distinct from any previously published sequences”
t0“Taxus baccata”
Claim · model-written summary
The AMF sequence types differed between the two study sites.
- Taxon
- Taxus baccataTaxus baccatat0
- Outcome
- differed between the two study sitese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differed between the two study sites”
t0“Taxus baccata”