Abstract
Antisera specific for the basic peroxidase from horseradish (Amoracea rusticana) were used to examine homology among horseradish peroxidase isoenzymes and among basic peroxidases from root plants. The antisera cross-reacted with all tested isoperoxidases when measured by both agar diffusion and quantitative precipitin reactions. Precipitin analyses provided quantitative measurements of homology among these plant peroxidases. The basic radish (Raphanus sativus L. cv. Cherry Belle) peroxidase had a high degree of homology (73 to 81%) with the basic peroxidase from horseradish. Turnip (Brassica rapa L. cv. Purple White Top Globe) and carrot (Daucus carota L. cv. Danvers) basic peroxidases showed less cross-reaction (49 to 54% and 41 to 46%, respectively). However, the cross-reactions of antisera with basic peroxidases from different plants were greater than were those observed with acidic horseradish isoenzymes (30 to 35%). These experiments suggest that basic peroxidase isoenzymes are strongly conserved during evolution and may indicate that the basic peroxidases catalyze reactions involved in specialized cellular functions. Anticatalytic assays were poor indicators of homology. Even though homology among isoperoxidases was detected by other immunological methods, antibodies inhibited only the catalytic activity of the basic peroxidase from radish.
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Claims 7
Claim · model-written summary
Antisera cross-reacted with all tested isoperoxidases.
Not reportedTaxon
Verbatim
e0“The antisera cross-reacted with all tested isoperoxidases when measured by both agar diffusion and quantitative precipitin reactions.”
Claim · model-written summary
Basic radish peroxidase showed 73 to 81% homology with basic horseradish peroxidase.
- Taxon
- radish
Raphanus sativuse0 - Gene
- peroxidasee0
- Outcome
- homologye0
- Value
- 73 to 81%e0
- Comparator
- horseradishe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The basic radish (Raphanus sativus L. cv. Cherry Belle) peroxidase had a high degree of homology (73 to 81%) with the basic peroxidase from horseradish.”
Claim · model-written summary
Turnip basic peroxidases showed 49 to 54% cross-reaction.
- Taxon
- Turnip
.jpg?width=60)
Brassica rapa subsp. rapae0 - Gene
- basic peroxidasese0
- Outcome
- cross-reactione0
- Direction
- lesse0
- Value
- 49 to 54%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Turnip (Brassica rapa L. cv. Purple White Top Globe) and carrot (Daucus carota L. cv. Danvers) basic peroxidases showed less cross-reaction (49 to 54% and 41 to 46%, respectively).”
Claim · model-written summary
Carrot basic peroxidases showed 41 to 46% cross-reaction.
- Taxon
- carrot
Daucus carotae0 - Gene
- basic peroxidasese0
- Outcome
- cross-reactione0
- Direction
- lesse0
- Value
- 41 to 46%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Turnip (Brassica rapa L. cv. Purple White Top Globe) and carrot (Daucus carota L. cv. Danvers) basic peroxidases showed less cross-reaction (49 to 54% and 41 to 46%, respectively).”
Claim · model-written summary
Basic peroxidase cross-reactions between plants exceeded acidic horseradish isoenzyme cross-reactions of 30 to 35%.
- Taxon
- horseradish
.jpg?width=60)
Armoracia rusticanae0 - Gene
- peroxidasese0
- Outcome
- cross-reactionse0
- Direction
- greatere0
- Value
- 30 to 35%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“However, the cross-reactions of antisera with basic peroxidases from different plants were greater than were those observed with acidic horseradish isoenzymes (30 to 35%).”
Claim · model-written summary
Anticatalytic assays were poor indicators of homology.
- Outcome
- indicators of homologye0
Not reportedComparatorValue
Verbatim
e0“Anticatalytic assays were poor indicators of homology.”
Claim · model-written summary
Antibodies inhibited catalytic activity only for basic radish peroxidase.
- Taxon
- radishRaphanus sativuse0
- Gene
- basic peroxidasee0
- Outcome
- catalytic activitye0
- Qualifier
- onlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Even though homology among isoperoxidases was detected by other immunological methods, antibodies inhibited only the catalytic activity of the basic peroxidase from radish.”