Abstract
Abstract Arrowleaf clover ( Trifolium vesiculosum Savi.) needs to be inoculated when sown in many fields in the southeastern USA. Survival of rhizobia on the seed following inoculation is critical for successful nodulation of the seedling. The effect the seed has on survival of the rhizobia has not been determined and may potentially be important to the success of inoculation. Seeds of subclover ( T. subterruneum L.) and different genotypes of arrowleaf clover were placed on inoculated yeast mannitol agar medium to determine if the seed contained diffusable substances toxic to rhizobia. A thermostable, watersoluble diffusate was released from autoclaved and nonautoclaved seeds that inhibited the growth of many strains of Rhizobium trifolii . Seed‐coat disinfecting techniques that utilized aqueous solutions reduced toxicity of the seed and indicated that method of seed disinfection should be taken into consideration when conducting experiments on survival of rhizobia on seed. Two selections of ‘Amclo’ arrowleaf clover were demonstrated not to be toxic to R. trifolii . Additional research should be undertaken to identify the toxic substances in the seed and to determine their influence on survival of rhizobia applied to seed as an inoculant. The nontoxic seed would serve as a good control for such experiments and may prove to be useful to plant breeders in determining the genetic basis of the toxic substances.
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Claims 4
Claim · model-written summary
A thermostable, water-soluble diffusate from arrowleaf clover seed inhibited the growth of many Rhizobium trifolii strains
- Taxon
- Trifolium vesiculosum
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Trifolium vesiculosume1 - Interactor
- Rhizobium trifolii
Rhizobium leguminosarume2symbiont - Outcome
- inhibited the growth of many strainse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“Arrowleaf clover ( Trifolium vesiculosum Savi.) needs to be inoculated when sown in many fields in the southeastern USA”
e2“A thermostable, watersoluble diffusate was released from autoclaved and nonautoclaved seeds that inhibited the growth of many strains of Rhizobium trifolii”
Claim · model-written summary
Seed-coat disinfection with aqueous solutions reduced the toxicity of arrowleaf clover seed
- Taxon
- Trifolium vesiculosumTrifolium vesiculosume1
- Treatment
- Seed‐coat disinfecting techniques that utilized aqueous solutionse3
- Outcome
- toxicity of the seede3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arrowleaf clover ( Trifolium vesiculosum Savi.) needs to be inoculated when sown in many fields in the southeastern USA”
e3“Seed‐coat disinfecting techniques that utilized aqueous solutions reduced toxicity of the seed”
Claim · model-written summary
Two selections of Amclo arrowleaf clover were not toxic to Rhizobium trifolii
- Taxon
- arrowleaf clovere4
- Material
- Amcloe4
- Interactor
- R. trifoliiRhizobium leguminosarume4symbiont
- Outcome
- not to be toxice4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Two selections of ‘Amclo’ arrowleaf clover were demonstrated not to be toxic to R. trifolii”
Claim · model-written summary
Non-toxic arrowleaf clover seed would serve as a good control for experiments on rhizobial survival
Verbatim
e1“Arrowleaf clover ( Trifolium vesiculosum Savi.) needs to be inoculated when sown in many fields in the southeastern USA”
e5“The nontoxic seed would serve as a good control for such experiments”