Abstract
Leucobryum boninense is endemic to the Bonin Islands, Japan, and its related species are widely distributed in Asia and the Pacific. We aimed to clarify the phylogenetic relationships among Leucobryum species and infer the origin of L. boninense. We also describe the utility of the chloroplast trnK intron including matK for resolving the phylogenetic relationships among Leucobryum species, as phylogenetic analyses using trnK intron and/or matK have not been performed well in bryophytes to date. Fifty samples containing 15 species of Leucobryum from Asia and the Pacific were examined for six chloroplast DNA regions including rbcL, rps4, partial 5' trnK intron, matK, partial 3' trnK intron, and trnL-F intergenic spacer plus one nuclear DNA region including ITS. A molecular phylogenetic tree showed that L. boninense made a clade with L. scabrum from Japan, Taiwan and, Hong Kong; L. javense which is widely distributed in East and Southeast Asia, and L. pachyphyllum and L. seemannii restricted to the Hawaii Islands, as well as with L. scaberulum from the Ryukyus, Japan, Taiwan, and southeastern China. Leucobryum boninense from various islands of the Bonin Islands made a monophylic group that was closely related to L. scabrum and L. javense from Japan. Therefore, L. boninense may have evolved from L. scabrum from Japan, Taiwan, or Hong Kong, or L. javense from Japan. We also described the utility of trnK intron including matK. A percentage of the parsimony-informative characters in trnK intron sequence data (5.8%) was significantly higher than that from other chloroplast regions, rbcL (2.4%) and rps4 (3.2%) sequence data. Nucleotide sequence data of the trnK intron including matK are more informative than other chloroplast DNA regions for identifying the phylogenetic relationships among Leucobryum species.
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Claims 6
Claim · model-written summary
L. boninense formed a clade with L. scabrum, L. javense, L. pachyphyllum, L. seemannii and L. scaberulum.
- Taxon
- L. boninenseLeucobryum boninensee0
- Outcome
- made a clade with L. scabrume0
Verbatim
e0“A molecular phylogenetic tree showed that L. boninense made a clade with L. scabrum from Japan, Taiwan and, Hong Kong; L. javense which is widely distributed in East and Southeast Asia, and L. pachyphyllum and L. seemannii restricted to the Hawaii Islands, as well as with L. scaberulum from the Ryukyus, Japan, Taiwan, and southeastern China”
Claim · model-written summary
Bonin Islands L. boninense formed a monophyletic group closely related to Japanese L. scabrum and L. javense.
- Taxon
- Leucobryum boninenseLeucobryum boninensee0
- Outcome
- made a monophylic group that was closely related to L. scabrum and L. javensee0
- Setting
- Bonin Islandse0
Verbatim
e0“Leucobryum boninense from various islands of the Bonin Islands made a monophylic group that was closely related to L. scabrum and L. javense from Japan”
Claim · model-written summary
L. boninense may have evolved from L. scabrum or L. javense.
- Taxon
- L. boninenseLeucobryum boninensee0
- Outcome
- may have evolved from L. scabrume0
- Setting
- Japan, Taiwan, or Hong Konge0
Not reportedDirection
Verbatim
e0“L. boninense may have evolved from L. scabrum from Japan, Taiwan, or Hong Kong, or L. javense from Japan”
Claim · model-written summary
The trnK intron had 5.8% parsimony-informative characters, significantly more than rbcL at 2.4%.
- Taxon
- Leucobryum
Leucobryumt0 - Outcome
- parsimony-informative characterse0
- Direction
- highere0
- Value
- 5.8%e0
- Qualifier
- 2.4%e0
- Comparator
- rbcLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A percentage of the parsimony-informative characters in trnK intron sequence data (5.8%) was significantly higher than that from other chloroplast regions, rbcL (2.4%) and rps4 (3.2%) sequence data”
t0“Leucobryum boninense”
Claim · model-written summary
The trnK intron had 5.8% parsimony-informative characters, significantly more than rps4 at 3.2%.
- Taxon
- LeucobryumLeucobryumt0
- Outcome
- parsimony-informative characterse0
- Direction
- highere0
- Value
- 5.8%e0
- Qualifier
- 3.2%e0
- Comparator
- rps4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A percentage of the parsimony-informative characters in trnK intron sequence data (5.8%) was significantly higher than that from other chloroplast regions, rbcL (2.4%) and rps4 (3.2%) sequence data”
t0“Leucobryum boninense”
Claim · model-written summary
trnK intron including matK sequence data were more informative than other chloroplast regions for Leucobryum phylogeny.
- Taxon
- LeucobryumLeucobryume0
- Outcome
- identifying the phylogenetic relationshipse0
- Direction
- more informativee0
- Comparator
- other chloroplast DNA regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nucleotide sequence data of the trnK intron including matK are more informative than other chloroplast DNA regions for identifying the phylogenetic relationships among Leucobryum species”