Abstract
ABSTRACTThe influence of genotype, explant's age and medium on in vitro cultivation of common bean (Phaseolus vulgaris L.) leaf petioles was investigated. Bulgarian cultivars Doubru-djanski 7; Plovdiv 11M and Plovdiv 10 were used in the experiments. Leaf petiole explants arising from in vitro seedlings were cultivated on modified MS media containing different amounts of TDZ, Paclobutrazol, NAA, BAP, IBA and GA3 in different combinations. Explant's development in different ages (5-; 7- and 14-days old) on the investigated media was genotype dependent. Bi-factorial ANOVA and three-factorial analyses were used for evaluation of the results that precisely disclosed the factor's interactions. The applied 3-factorial dispersion analysis revealed that the interaction between factors genotype-explant age (AB) and the threefold interaction genotype-explant age-media (ABC) exerted the strongest influence on the explant development and callus formation. The influence of interaction between factors genotype-explant's age (AB) increase along with cultivation time. No dependence was found between the size of initial plants, leaf petiole position (near to the leaf or to the steam), explant fresh weight, their development and quantity of formed callus. An additional experiment with precultivated sterile seeds on media containing 1 μM IAA or 1 μM BAP (on the base of MS) contributed. Better explant development was observed in comparison to their cultivation only on MS medium.
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Claims 5
Claim · model-written summary
Explant development across tested ages and media depended on genotype.
- Taxon
- common bean
Phaseolus vulgarist0 - Material
- genotypee0
- Outcome
- Explant's developmente0
- Qualifier
- 5-; 7- and 14-days olde0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Explant's development in different ages (5-; 7- and 14-days old) on the investigated media was genotype dependent.”
t0“Common Bean”
Claim · model-written summary
Genotype-age and genotype-age-media interactions most strongly influenced development and callus formation.
- Taxon
- common beanPhaseolus vulgarist0
- Material
- genotype-explant age (AB)e0
- Outcome
- explant development and callus formatione0
- Direction
- strongeste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the interaction between factors genotype-explant age (AB) and the threefold interaction genotype-explant age-media (ABC) exerted the strongest influence on the explant development and callus formation.”
t0“Common Bean”
Claim · model-written summary
The genotype-age interaction increased over cultivation time.
- Taxon
- common beanPhaseolus vulgarist0
- Material
- genotype-explant's age (AB)e0
- Outcome
- influence of interactione0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The influence of interaction between factors genotype-explant's age (AB) increase along with cultivation time.”
t0“Common Bean”
Claim · model-written summary
Initial plant size, petiole position, and explant fresh weight had no detected relation with development or callus quantity.
- Taxon
- common beanPhaseolus vulgarist0
- Organ / stage
- leaf petiolee0
- Outcome
- their development and quantity of formed calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No dependence was found between the size of initial plants, leaf petiole position (near to the leaf or to the steam), explant fresh weight, their development and quantity of formed callus.”
t0“Common Bean”
Claim · model-written summary
Seed precultivation in IAA or BAP improved explant development compared with MS alone.
- Taxon
- common beanPhaseolus vulgarist0
- Treatment
- 1 μM IAA or 1 μM BAPe0
- Outcome
- explant developmente0
- Direction
- Bettere0
- Comparator
- MS mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“precultivated sterile seeds on media containing 1 μM IAA or 1 μM BAP (on the base of MS) contributed. Better explant development was observed in comparison to their cultivation only on MS medium.”
t0“Common Bean”
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