Abstract
Culture of cassava somatic embryos on media with an altered macro- and micro-nutrient salt concentration affected embryo development and germination capability. In the tests, quarter-, half-, full- or double-strength Murashige and Skoog (MS) media were compared. The maximum number of somatic embryos differentiated from a proliferative nodular embryogenic callus (NEC) on either half- or full-strength MS medium, and the greatest numbers of cotyledonary stage embryos were formed on full-strength MS medium. Developed somatic embryos were then desiccated above a saturated K2SO4 solution for 10 d. After transfer to germination medium, embryos that had developed on half- and full-strength MS medium yielded 8.3 and 8.6 germinants g(-1) NEC tissue, respectively. For this important but often disregarded culture factor, either half- or full-strength MS medium is recommended for both the differentiation and development of cassava somatic embryos that are capable of germination.
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Claims 5
Claim · model-written summary
Half-strength MS medium produced the maximum number of differentiated cassava somatic embryos.
- Taxon
- cassava
Manihot esculentat0 - Organ / stage
- somatic embryose0
- Treatment
- half- or full-strength MS mediume0
- Outcome
- number of somatic embryos differentiatede0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum number of somatic embryos differentiated from a proliferative nodular embryogenic callus (NEC) on either half- or full-strength MS medium”
t0“Cassava”
Claim · model-written summary
Full-strength MS medium produced the greatest number of cotyledonary stage cassava embryos.
- Taxon
- cassavaManihot esculentat0
- Treatment
- full-strength MS mediume0
- Outcome
- numbers of cotyledonary stage embryose0
- Direction
- greateste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the greatest numbers of cotyledonary stage embryos were formed on full-strength MS medium”
t0“Cassava”
Claim · model-written summary
Embryos developed on half-strength MS medium yielded 8.3 germinants per gram of NEC tissue.
- Taxon
- cassavaManihot esculentat0
- Treatment
- half- and full-strength MS mediume0
- Outcome
- germinantse0
- Value
- 8.3e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“embryos that had developed on half- and full-strength MS medium yielded 8.3 and 8.6 germinants g(-1) NEC tissue, respectively”
t0“Cassava”
Claim · model-written summary
Embryos developed on full-strength MS medium yielded 8.6 germinants per gram of NEC tissue.
- Taxon
- cassavaManihot esculentat0
- Treatment
- half- and full-strength MS mediume0
- Outcome
- germinantse0
- Value
- 8.6 germinants g(-1) NEC tissuee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“embryos that had developed on half- and full-strength MS medium yielded 8.3 and 8.6 germinants g(-1) NEC tissue, respectively”
t0“Cassava”
Claim · model-written summary
The authors recommend half- or full-strength MS medium for differentiating and developing germination-capable cassava somatic embryos.
- Taxon
- cassavaManihot esculentae0
- Organ / stage
- cassava somatic embryose0
- Outcome
- half- or full-strength MS mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“either half- or full-strength MS medium is recommended for both the differentiation and development of cassava somatic embryos that are capable of germination”
The same questions in other papers
in vitro · growth · Manihot esculenta (cassava)Positive and negative results
29 papers in the same direction as this paper · 5 in the opposite direction
- positive
- negative
- no effect
31 papers more in this group
See all 35 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.