Paper · 2009 · Biodiversity Research and Conservation

Genetic diversity of Avena strigosa Schreb. ecotypes on the basis of isoenzyme markers

Katarzyna Kubiak

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Open access · cc-by-nc-nd

Abstract

Genetic diversity of Avena strigosa Schreb. ecotypes on the basis of isoenzyme markers Genetic diversity was analyzed in 19 ecotypes of the diploid oat A. strigosa originating from various geographical regions of the world. Six isoenzyme systems (AAT, ACP, EST, LAP, MDH, PX) were studied and 16 loci were identified. Only two loci ( Est4 and Mdh2 ) were polymorphic. Ecotypes were characterized by the percentage of polymorphic loci (P=3.3%), the mean number of alleles per locus (A=1.04) and intrapopulation diversity (H S =0.013). Total genetic diversity (H T =0.07) and interpopulation diversity (D ST =0.057) were examined as well. The value of the coefficient of gene differentiation (G ST =0.821) indicated that diversity among populations was an important contributor to total variability. Genetic similarity between A. strigosa populations was very high (I N =0.94). Cluster analysis did not demonstrate strongly differentiated groups among the ecotypes examined.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

Six isoenzyme systems yielded 16 identified loci.

Outcome
16 loci were identifiede0
Value
16 locie0

Not reportedTaxon

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e0

“Six isoenzyme systems (AAT, ACP, EST, LAP, MDH, PX) were studied and 16 loci were identified”

✓ located verbatim in the source · abstract, characters 224–316 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Only Est4 and Mdh2 were polymorphic.

Gene
Est4 and Mdh2e0
Outcome
polymorphice0
Value
two locie0

Not reportedTaxon

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e0

“Only two loci ( Est4 and Mdh2 ) were polymorphic”

✓ located verbatim in the source · abstract, characters 318–366 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Polymorphic loci comprised 3.3%.

Outcome
percentage of polymorphic locie0
Value
3.3%e0

Not reportedTaxon

Verbatim

e0

“percentage of polymorphic loci (P=3.3%)”

✓ located verbatim in the source · abstract, characters 403–442 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The mean number of alleles per locus was 1.04.

Outcome
mean number of alleles per locuse0
Value
1.04e0

Not reportedTaxon

Verbatim

e0

“mean number of alleles per locus (A=1.04)”

✓ located verbatim in the source · abstract, characters 448–489 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Intrapopulation diversity was 0.013.

Outcome
intrapopulation diversitye0
Value
0.013e0

Not reportedTaxon

Verbatim

e0

“intrapopulation diversity (H S =0.013)”

✓ located verbatim in the source · abstract, characters 494–532 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Total genetic diversity was 0.07.

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Total genetic diversitye0
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0.07e0

Not reportedTaxon

Verbatim

e0

“Total genetic diversity (H T =0.07)”

✓ located verbatim in the source · abstract, characters 534–569 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Interpopulation diversity was 0.057.

Outcome
interpopulation diversitye0
Value
0.057e0

Not reportedTaxon

Verbatim

e0

“interpopulation diversity (D ST =0.057)”

✓ located verbatim in the source · abstract, characters 574–613 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Among-population diversity was an important contributor to total variability, with differentiation coefficient 0.821.

Outcome
diversity among populations was an important contributor to total variabilitye0
Value
0.821e0

Not reportedTaxon

Verbatim

e0

“coefficient of gene differentiation (G ST =0.821) indicated that diversity among populations was an important contributor to total variability”

✓ located verbatim in the source · abstract, characters 654–796 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Genetic similarity between populations was very high at 0.94.

Taxon
A. strigosaAvena strigosae0
Outcome
Genetic similaritye0
Direction
very highe0
Value
0.94e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena strigosa
Avena strigosa · TaxonMiquel Pujol Palol · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Genetic similarity between A. strigosa populations was very high (I N =0.94)”

✓ located verbatim in the source · abstract, characters 798–874 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cluster analysis did not show strongly differentiated ecotype groups.

Outcome
did not demonstrate strongly differentiated groupse0

Not reportedTaxon

Verbatim

e0

“Cluster analysis did not demonstrate strongly differentiated groups among the ecotypes examined”

✓ located verbatim in the source · abstract, characters 876–971 · show in text ↑