Immunochemical Approach for Analysis of Stress Response in Wheat Seedlings Affected by Different External Factors

Abstract

An immunochemical approach for comparative analysis of protein changes in wheat seedlings subjected to different stress factors was developed. It is based on the production of antibodies against a total protein extract from roots of cold stressed wheat seedlings with the presumption for a high prevailance of the stress proteins in the early stages of the cold stress response due to the reduced rate of the overall physiological processes. The polyclonal antiserum was affinity purified and enriched by filtering against a total protein extract from control seedlings. The western blot analysis revealed protein profiles specific for the applied stress factors. Thus, the method allowes simultaneous analysis of different stress factors. It is quick and sensitive and can be suggested as a preliminary test in selection of stress tolerant agriculture plants and as an alternative to the use of radiolabeled compounds.

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