Abstract
Crown gall tumor formation involves integration into the plant genome of DNA sequences (the T-region) of tumor-inducing (Ti) plasmids present in Agrobacterium tumefaciens. The T-DNA of the tumor expresses several gene products. Little is known about the function or regulation of expression of the 0.7kb transcript, which represents a relatively abundant T-DNA transcript in octopine-type tumors. In this report, a detailed structural analysis of the gene encoding the 0.7 kb transcript has been obtained by DNA sequence analysis of T-DNA isolated from A6S/2 tumor line. An indication of the structural characteristics of the protein product is obtained from the predicted amino acid sequence. The sequences flanking the open reading frame show characteristics with other eucaryotic genes. The corresponding DNA sequence of the inducing Ti plasmid (pTiA6) is identical with that of the DNA sequence from the tumor. Comparison of this gene sequence with the corresponding region of another Ti plasmid (pTiAch5) shows several differences in the 5' flanking sequence, but the nucleotide sequence of the coding region and 3' flanking region are identical.
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Claims 4
Claim · model-written summary
Structural analysis of the gene encoding the 0.7 kb transcript was obtained from T-DNA of the A6S/2 tumour line.
- Taxon
- Agrobacterium tumefaciens
Agrobacterium tumefaciense0 - Material
- A6S/2 tumor linee1
- Gene
- 0.7 kb transcripte1
- Outcome
- structural analysis of the gene encoding the 0.7 kb transcripte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tumor-inducing (Ti) plasmids present in Agrobacterium tumefaciens”
e1“a detailed structural analysis of the gene encoding the 0.7 kb transcript has been obtained by DNA sequence analysis of T-DNA isolated from A6S/2 tumor line”
Claim · model-written summary
The DNA sequence of the inducing Ti plasmid pTiA6 is identical to the T-DNA sequence from the tumour.
- Taxon
- Agrobacterium tumefaciensAgrobacterium tumefaciense0
- Outcome
- DNA sequence of the inducing Ti plasmid (pTiA6)e2
- Qualifier
- identical with that of the DNA sequence from the tumore2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tumor-inducing (Ti) plasmids present in Agrobacterium tumefaciens”
e2“The corresponding DNA sequence of the inducing Ti plasmid (pTiA6) is identical with that of the DNA sequence from the tumor”
Claim · model-written summary
The gene sequence differs from that of Ti plasmid pTiAch5 in several places in the 5' flanking sequence.
- Taxon
- Agrobacterium tumefaciensAgrobacterium tumefaciense0
- Outcome
- 5' flanking sequencee3
- Qualifier
- several differencese3
- Comparator
- pTiAch5e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tumor-inducing (Ti) plasmids present in Agrobacterium tumefaciens”
e3“Comparison of this gene sequence with the corresponding region of another Ti plasmid (pTiAch5) shows several differences in the 5' flanking sequence”
Claim · model-written summary
The coding region and 3' flanking region of the gene are identical to those of Ti plasmid pTiAch5.
- Taxon
- Agrobacterium tumefaciensAgrobacterium tumefaciense0
- Outcome
- nucleotide sequence of the coding region and 3' flanking regione4
- Qualifier
- identicale4
- Comparator
- pTiAch5e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tumor-inducing (Ti) plasmids present in Agrobacterium tumefaciens”
e3“Comparison of this gene sequence with the corresponding region of another Ti plasmid (pTiAch5) shows several differences in the 5' flanking sequence”
e4“the nucleotide sequence of the coding region and 3' flanking region are identical”