Paper · 1991 · Crop Science

Analysis of C‐Banding Chromosome Patterns of Sorghum

Hong Yu; George H. Liang; Kenneth D. Kofoid

8
claims
16
verbatim quotes
18
citations
1
research areas
—
Not open access

Abstract

Chromosome banding patterns can reveal the locations of specific heterochromatin characteristics for individual chromosomes in a given species. Thus, banding patterns have been used to identify individual chromosomes, to detect chromosomal structural aberrations, and to elucidate evolutionary relationships among taxonomically related species. However, such banding patterns have not been documented for any of the species in the genus Sorghum . Since these chromosomes are small and frequently difficult to distinguish, the C‐banding technique was applied to Combine Kafir 60, a cultivar of Sorghum bicolor (L.) Moench, to determine the banding patterns of the chromosome complement. While different C‐banding treatments gave somewhat different karyotypes, all gave satisfactory results for identifying individual chromosomes. All chromosomes of Combine Kafir 60 showed intensive centromeric C‐bands. Intercalary bands were present in Chromosomes 3, 4, 5, and 9, with some variations in their positions. Telomeric C‐bands were identified in both arms of Chromosomes 1, 2, and 7, and in the short arm of Chromosome 3. Chromosomes 6, 8, and 10 had only centrometic Cbands. Some C‐bands were constant in showing their characteristics and some were facultative. All 10 chromosome pairs in Combine Kafir 60 were identified by their C‐banding patterns, along with their length and arm ratios. This banding pattern can be used as the basis for detecting intraspecific chromosomal variability and an interspecific genomic relationship.

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Claims 8

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The C-banding technique gave satisfactory results for identifying individual chromosomes of Combine Kafir 60

Taxon
Sorghum bicolorSorghum bicolore1
Material
Combine Kafir 60e1
Outcome
identifying individual chromosomese2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Combine Kafir 60, a cultivar of Sorghum bicolor (L.) Moench”

✓ located verbatim in the source · abstract, characters 561–620 · show in text ↑
e2

“all gave satisfactory results for identifying individual chromosomes”

✓ located verbatim in the source · abstract, characters 759–827 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

All chromosomes of Combine Kafir 60 showed intensive centromeric C-bands

Taxon
Sorghum bicolorSorghum bicolore1
Material
Combine Kafir 60e3
Outcome
intensive centromeric C‐bandse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Combine Kafir 60, a cultivar of Sorghum bicolor (L.) Moench”

✓ located verbatim in the source · abstract, characters 561–620 · show in text ↑
e3

“All chromosomes of Combine Kafir 60 showed intensive centromeric C‐bands”

✓ located verbatim in the source · abstract, characters 829–901 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Intercalary C-bands were present in chromosomes 3, 4, 5 and 9 of Combine Kafir 60

Taxon
Sorghum bicolorSorghum bicolore1
Material
Combine Kafir 60e1
Outcome
Intercalary bandse4
Qualifier
Chromosomes 3, 4, 5, and 9e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Combine Kafir 60, a cultivar of Sorghum bicolor (L.) Moench”

✓ located verbatim in the source · abstract, characters 561–620 · show in text ↑
e4

“Intercalary bands were present in Chromosomes 3, 4, 5, and 9, with some variations in their positions”

✓ located verbatim in the source · abstract, characters 903–1004 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Telomeric C-bands were present in both arms of chromosomes 1, 2 and 7 and the short arm of chromosome 3

Taxon
Sorghum bicolorSorghum bicolore1
Material
Combine Kafir 60e1
Outcome
Telomeric C‐bandse5
Qualifier
both arms of Chromosomes 1, 2, and 7e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Combine Kafir 60, a cultivar of Sorghum bicolor (L.) Moench”

✓ located verbatim in the source · abstract, characters 561–620 · show in text ↑
e5

“Telomeric C‐bands were identified in both arms of Chromosomes 1, 2, and 7, and in the short arm of Chromosome 3”

✓ located verbatim in the source · abstract, characters 1006–1117 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Chromosomes 6, 8 and 10 had only centromeric C-bands

Taxon
Sorghum bicolorSorghum bicolore1
Material
Combine Kafir 60e1
Outcome
only centrometic Cbandse6
Qualifier
Chromosomes 6, 8, and 10e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Combine Kafir 60, a cultivar of Sorghum bicolor (L.) Moench”

✓ located verbatim in the source · abstract, characters 561–620 · show in text ↑
e6

“Chromosomes 6, 8, and 10 had only centrometic Cbands”

✓ located verbatim in the source · abstract, characters 1119–1171 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Some C-bands of Combine Kafir 60 were constant in their characteristics and some were facultative

Taxon
Sorghum bicolorSorghum bicolore1
Material
Combine Kafir 60e1
Outcome
Some C‐bands were constant in showing their characteristicse7
Qualifier
some were facultativee7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Combine Kafir 60, a cultivar of Sorghum bicolor (L.) Moench”

✓ located verbatim in the source · abstract, characters 561–620 · show in text ↑
e7

“Some C‐bands were constant in showing their characteristics and some were facultative”

✓ located verbatim in the source · abstract, characters 1173–1258 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

All 10 chromosome pairs of Combine Kafir 60 were identified by their C-banding patterns

Taxon
Sorghum bicolorSorghum bicolore1
Material
Combine Kafir 60e0
Outcome
identified by their C‐banding patternse0
Qualifier
along with their length and arm ratiose0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Combine Kafir 60, a cultivar of Sorghum bicolor (L.) Moench”

✓ located verbatim in the source · abstract, characters 561–620 · show in text ↑
e0

“All 10 chromosome pairs in Combine Kafir 60 were identified by their C‐banding patterns, along with their length and arm ratios. This banding pattern can be used as the basis for detecting intraspecific chromosomal variability and an interspecific genomic relationship”

✓ located verbatim in the source · abstract, characters 1260–1528 · show in text ↑
Observationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The C-banding pattern could be used to detect intraspecific chromosomal variability and interspecific genomic relationship in sorghum

Taxon
Sorghum bicolorSorghum bicolore1
Outcome
detecting intraspecific chromosomal variability and an interspecific genomic relationshipe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Combine Kafir 60, a cultivar of Sorghum bicolor (L.) Moench”

✓ located verbatim in the source · abstract, characters 561–620 · show in text ↑
e0

“All 10 chromosome pairs in Combine Kafir 60 were identified by their C‐banding patterns, along with their length and arm ratios. This banding pattern can be used as the basis for detecting intraspecific chromosomal variability and an interspecific genomic relationship”

✓ located verbatim in the source · abstract, characters 1260–1528 · show in text ↑