Abstract
In conventional photosystem II preparation high amounts of Cu are found. After fractionation by centrifugation, Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins. We could demonstrate that the Cu was associated with nuclear contaminants in the starch fraction. Among the contaminants, several histones were identified by specific antisera and by N‐terminal sequencing. In order to obtain homogeneous BBY preparations of PSII a procedure is employed that involves a 10000 g centrifugation step and which eliminates non‐specifically bound metals, nucleic acids and histones with the starch pellet. The resulting starch‐free BBY (BBY s ‐), which is free of these nuclear contaminants, is an appropriate preparation for biophysical studies or for those of metal interactions with PSII.
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Claims 5
Claim · model-written summary
Copper can be removed from photosystem II without affecting its photosynthetic activity or the composition of its specific proteins.
- Outcome
- photosynthetic activity or the composition of its specific proteinse0
- Comparator
- conventional photosystem II preparatione1
Not reportedTaxon
Verbatim
e0“Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins”
e1“In conventional photosystem II preparation high amounts of Cu are found”
Claim · model-written summary
Cu was associated with nuclear contaminants in the starch fraction of PSII preparations.
Not reportedTaxon
Verbatim
e2“We could demonstrate that the Cu was associated with nuclear contaminants in the starch fraction”
Claim · model-written summary
Several histones were identified among the nuclear contaminants of PSII preparations.
Not reportedTaxon
Verbatim
e3“several histones were identified by specific antisera”
Claim · model-written summary
A 10000 g centrifugation step eliminates nucleic acids and histones with the starch pellet.
Not reportedTaxon
Verbatim
e4“a procedure is employed that involves a 10000 g centrifugation step”
e5“nucleic acids and histones with the starch pellet”
Claim · model-written summary
The starch-free BBY preparation is an appropriate preparation for biophysical studies and for metal-interaction studies with PSII.
- Outcome
- appropriate preparation for biophysical studiese6
Not reportedTaxon
Verbatim
e6“is an appropriate preparation for biophysical studies”