Paper · 1983 · Plant Pathology

Resistance to benzimidazole fungicides in the gummy stem blight pathogen Didymelta bryoniae on cucurbits

N. E. MALATHRAKIS; D. J. VAKALOUNAKIS

8
claims
23
verbatim quotes
27
citations
1
research areas
—
Not open access

Abstract

Benzimidazoles were used successfully against gummy stem blight of cucurbits for only one growing season (1980). It was soon noticed in experiments in vivo that they were not effective against some isolates of the pathogen. In a survey during the 1981 crop season, 76 out of 102 isolates collected from 20 plastic greenhouses all over the main cucurbit‐growing areas of Crete were resistant to benomyl. The average benomyl concentrations that reduced mycelial growth rates of seven resistant and seven sensitive isolates by 50% were 716 ± 49 and 1.2 ± 0.34μg/ml respectively. The average growth rate of resistant isolates was lower than that of sensitive isolates but there were no differences in spore production and spore germination. The pathogenicity of resistant isolates on young cucumber plants was lower than that of sensitive isolates. The application of benomyl, carbendazim, cypendazole and thiophanate methyl to young cucumber plants at the rate of 300μg a.i./ml 1 day before inoculation with 500 000 spores/ml of the resistant isolates did not affect the infection rate of the disease after 4 days. Sensitive isolates caused no onslight symptoms on similarly treated plants.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

In a survey of Cretan greenhouses, 76 of 102 Didymelta bryoniae isolates were resistant to benomyl.

Taxon
cucurbitse0
Interactor
Didymelta bryoniaet0pathogen
Outcome
resistant to benomyle1
Value
76 out of 102e1
Setting
plastic greenhousese1

Verbatim

e0

“Benzimidazoles were used successfully against gummy stem blight of cucurbits for only one growing season (1980)”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e1

“76 out of 102 isolates collected from 20 plastic greenhouses all over the main cucurbit‐growing areas of Crete were resistant to benomyl”

✓ located verbatim in the source · abstract, characters 265–401 · show in text ↑
t0

“gummy stem blight pathogen Didymelta bryoniae”

✓ located verbatim in the source · title, characters 46–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Resistant Didymelta bryoniae isolates needed an average benomyl concentration of 716 ± 49 to halve mycelial growth rate.

Taxon
cucurbitse0
Material
resistant isolatese3
Interactor
Didymelta bryoniaet0pathogen
Outcome
benomyl concentrations that reduced mycelial growth ratese2
Value
716 ± 49e2

Verbatim

e0

“Benzimidazoles were used successfully against gummy stem blight of cucurbits for only one growing season (1980)”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e2

“The average benomyl concentrations that reduced mycelial growth rates of seven resistant and seven sensitive isolates by 50% were 716 ± 49 and 1.2 ± 0.34μg/ml respectively”

✓ located verbatim in the source · abstract, characters 403–574 · show in text ↑
e3

“The average growth rate of resistant isolates was lower than that of sensitive isolates but there were no differences in spore production and spore germination”

✓ located verbatim in the source · abstract, characters 576–735 · show in text ↑
t0

“gummy stem blight pathogen Didymelta bryoniae”

✓ located verbatim in the source · title, characters 46–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Sensitive Didymelta bryoniae isolates needed an average benomyl concentration of 1.2 ± 0.34 to halve mycelial growth rate.

Taxon
cucurbitse0
Material
sensitive isolatese3
Interactor
Didymelta bryoniaet0pathogen
Outcome
benomyl concentrations that reduced mycelial growth ratese2
Value
1.2 ± 0.34e2

Verbatim

e0

“Benzimidazoles were used successfully against gummy stem blight of cucurbits for only one growing season (1980)”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e2

“The average benomyl concentrations that reduced mycelial growth rates of seven resistant and seven sensitive isolates by 50% were 716 ± 49 and 1.2 ± 0.34μg/ml respectively”

✓ located verbatim in the source · abstract, characters 403–574 · show in text ↑
e3

“The average growth rate of resistant isolates was lower than that of sensitive isolates but there were no differences in spore production and spore germination”

✓ located verbatim in the source · abstract, characters 576–735 · show in text ↑
t0

“gummy stem blight pathogen Didymelta bryoniae”

✓ located verbatim in the source · title, characters 46–91 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Resistant isolates had a lower mycelial growth rate than sensitive isolates.

Taxon
cucurbitse0
Material
resistant isolatese3
Interactor
Didymelta bryoniaet0pathogen
Outcome
growth ratee3
Direction
lowere3
Comparator
sensitive isolatese3

Verbatim

e0

“Benzimidazoles were used successfully against gummy stem blight of cucurbits for only one growing season (1980)”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e3

“The average growth rate of resistant isolates was lower than that of sensitive isolates but there were no differences in spore production and spore germination”

✓ located verbatim in the source · abstract, characters 576–735 · show in text ↑
t0

“gummy stem blight pathogen Didymelta bryoniae”

✓ located verbatim in the source · title, characters 46–91 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Resistant isolates were less pathogenic on young cucumber plants than sensitive isolates.

Taxon
cucumberCucumis sativuse4
Material
resistant isolatese4
Interactor
Didymelta bryoniaet0pathogen
Outcome
pathogenicitye4
Direction
lowere4
Comparator
sensitive isolatese4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“The pathogenicity of resistant isolates on young cucumber plants was lower than that of sensitive isolates”

✓ located verbatim in the source · abstract, characters 737–843 · show in text ↑
t0

“gummy stem blight pathogen Didymelta bryoniae”

✓ located verbatim in the source · title, characters 46–91 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

Resistant and sensitive isolates did not differ in spore production or spore germination.

Taxon
cucurbitse0
Material
resistant isolatese3
Outcome
spore production and spore germinatione3
Direction
no differencese3
Comparator
sensitive isolatese3

Verbatim

e0

“Benzimidazoles were used successfully against gummy stem blight of cucurbits for only one growing season (1980)”

✓ located verbatim in the source · abstract, characters 0–111 · show in text ↑
e3

“The average growth rate of resistant isolates was lower than that of sensitive isolates but there were no differences in spore production and spore germination”

✓ located verbatim in the source · abstract, characters 576–735 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Benomyl, carbendazim, cypendazole and thiophanate methyl applied at 300μg a.i./ml one day before inoculation did not affect infection by resistant isolates on cucumber.

Taxon
cucumberCucumis sativuse5
Treatment
benomyl, carbendazim, cypendazole and thiophanate methyle5
Interactor
Didymelta bryoniaet0pathogen
Outcome
infection ratee5
Direction
did not affecte5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e5

“The application of benomyl, carbendazim, cypendazole and thiophanate methyl to young cucumber plants at the rate of 300μg a.i./ml 1 day before inoculation with 500 000 spores/ml of the resistant isolates did not affect the infection rate of the disease after 4 days”

✓ located verbatim in the source · abstract, characters 845–1110 · show in text ↑
t0

“gummy stem blight pathogen Didymelta bryoniae”

✓ located verbatim in the source · title, characters 46–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Sensitive isolates caused no or only slight symptoms on cucumber plants treated with the benzimidazoles.

Taxon
cucumberCucumis sativuse4
Material
Sensitive isolatese6
Interactor
Didymelta bryoniaet0pathogen
Outcome
symptomse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“The pathogenicity of resistant isolates on young cucumber plants was lower than that of sensitive isolates”

✓ located verbatim in the source · abstract, characters 737–843 · show in text ↑
e6

“Sensitive isolates caused no onslight symptoms on similarly treated plants”

✓ located verbatim in the source · abstract, characters 1112–1186 · show in text ↑
t0

“gummy stem blight pathogen Didymelta bryoniae”

✓ located verbatim in the source · title, characters 46–91 · show in text ↑

carbendazim · disease · Cucumis sativus (cucumber)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

benomyl · disease · Cucumis sativus (cucumber)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

2 in the opposite direction

  1. positive
  2. positive
See all 3 papers →

thiophanate-methyl · disease · Cucumis sativus (cucumber)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.