Abstract
Northern blot analysis is a powerful research tool for discovery, validation and expression of genes, and is currently widely used to detect microRNA (miRNA) accumulation. However, the traditional Northern blot procedure, which is based on a support membrane, is overly elaborate and time-consuming, although it is unsurpassed in accuracy for determining the sizes and amounts of multiple small RNAs sharing high sequence identity. Here we present an alternative method derived from plant miRNAs, liquid Northern hybridization, using fluorescently labeled oligonucleotide probes and characterized by simple and specific miRNA determination and quantitation. The entire detection process is completed within a few hours, and multiple miRNAs can be simultaneously detected in a single experiment.
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Claims 3
Claim · model-written summary
Liquid Northern hybridization permits simple and specific miRNA determination and quantitation.
Not reportedComparator
Verbatim
e0“liquid Northern hybridization, using fluorescently labeled oligonucleotide probes and characterized by simple and specific miRNA determination and quantitation”
t0“plant miRNAs”
Claim · model-written summary
The detection process takes a few hours.
Verbatim
e0“The entire detection process is completed within a few hours”
t0“plant miRNAs”
Claim · model-written summary
Multiple miRNAs can be detected simultaneously in one experiment.
Verbatim
e0“multiple miRNAs can be simultaneously detected in a single experiment”
t0“plant miRNAs”