Abstract
The microsymbiont population in soybean root nodules (Glycine max L. cv Williams 82 inoculated with Bradyrhizobium japonicum 2143) was characterized during symbiotic development to determine the extent of heterogeneity in this population. The microsymbiont population was isolated by centrifugation through a continuous sucrose gradient (44 to 57% weight to weight ratio) and appeared homogeneous at each age examined up to 26 days after planting based on the symmetrical distribution of the population, enzyme activities, poly-beta-hydroxybutyrate contents, protein contents, and viabilities. Some differences in viability, protein content, and acetylene reduction activity were observed at later ages. The population migrated to progressively lighter buoyant densities with increasing age until a density equivalent to 48% sucrose was reached. The changing density correlated directly with the increasing poly-beta-hydroxybutyrate to protein ratio. The acetylene reduction activity, based on microsymbiont concentration, followed the same developmental pattern as whole nodules. On a protein basis, the decline of acetylene reduction activity was later and reflected the decrease in protein content per cell. These results suggested that the microsymbiont population, which resulted from inoculation of B. japonicum 2143 onto Williams 82 cultivar of soybeans, developed as a homogeneous population.
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Claims 6
Claim · model-written summary
The microsymbiont population appeared homogeneous at each age examined up to 26 days after planting.
- Taxon
- soybean
Glycine maxe0 - Material
- Williams 82e0
- Interactor
- Bradyrhizobium japonicum 2143
Bradyrhizobium japonicume0symbiont - Outcome
- homogeneouse1
- Value
- 26 dayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean root nodules (Glycine max L. cv Williams 82 inoculated with Bradyrhizobium japonicum 2143)”
e1“appeared homogeneous at each age examined up to 26 days after planting”
Claim · model-written summary
Viability, protein content, and acetylene reduction activity differed at later ages.
- Taxon
- soybeanGlycine maxe0
- Interactor
- Bradyrhizobium japonicum 2143Bradyrhizobium japonicume0symbiont
- Outcome
- differences in viability, protein content, and acetylene reduction activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean root nodules (Glycine max L. cv Williams 82 inoculated with Bradyrhizobium japonicum 2143)”
e1“Some differences in viability, protein content, and acetylene reduction activity were observed at later ages”
Claim · model-written summary
The microsymbiont population moved to lighter buoyant densities with age until the density equivalent to 48% sucrose.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- increasing agee1
- Interactor
- Bradyrhizobium japonicum 2143Bradyrhizobium japonicume0symbiont
- Outcome
- buoyant densitiese1
- Direction
- lightere1
- Value
- 48% sucrosee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean root nodules (Glycine max L. cv Williams 82 inoculated with Bradyrhizobium japonicum 2143)”
e1“The population migrated to progressively lighter buoyant densities with increasing age until a density equivalent to 48% sucrose was reached”
Claim · model-written summary
Changing microsymbiont density correlated directly with the increasing poly-beta-hydroxybutyrate to protein ratio.
- Taxon
- soybeanGlycine maxe0
- Interactor
- Bradyrhizobium japonicum 2143Bradyrhizobium japonicume0symbiont
- Compound
- poly-beta-hydroxybutyratee1
- Outcome
- changing densitye1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean root nodules (Glycine max L. cv Williams 82 inoculated with Bradyrhizobium japonicum 2143)”
e1“The changing density correlated directly with the increasing poly-beta-hydroxybutyrate to protein ratio”
Claim · model-written summary
Microsymbiont acetylene reduction activity per concentration followed the developmental pattern of whole nodules.
- Taxon
- soybeanGlycine maxe0
- Interactor
- Bradyrhizobium japonicum 2143Bradyrhizobium japonicume0symbiont
- Outcome
- acetylene reduction activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean root nodules (Glycine max L. cv Williams 82 inoculated with Bradyrhizobium japonicum 2143)”
e1“The acetylene reduction activity, based on microsymbiont concentration, followed the same developmental pattern as whole nodules”
Claim · model-written summary
On a protein basis, acetylene reduction activity declined later, reflecting decreased protein content per cell.
- Taxon
- soybeanGlycine maxe0
- Interactor
- Bradyrhizobium japonicum 2143Bradyrhizobium japonicume0symbiont
- Outcome
- acetylene reduction activitye1
- Direction
- declinee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean root nodules (Glycine max L. cv Williams 82 inoculated with Bradyrhizobium japonicum 2143)”
e1“On a protein basis, the decline of acetylene reduction activity was later and reflected the decrease in protein content per cell”