Abstract
A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR.
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Claims 10
Claim · model-written summary
A virus was isolated from soybean plants showing dwarfing and bud blight.
- Taxon
- soybean
Glycine maxe0 - Interactor
- A viruse0pathogen
- Outcome
- symptoms of dwarfing and bud blighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight”
Claim · model-written summary
The isolate had a host range and properties resembling Tobacco streak virus.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Tobacco streak virus (TSV)
Tobacco streak viruse0pathogen - Outcome
- host range and propertiese0
- Comparator
- Tobacco streak virus (TSV)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“host range and properties resembled those of Tobacco streak virus (TSV)”
e1“soybean (Glycine max)”
Claim · model-written summary
Purified soybean virus formed three peaks in a frozen sucrose gradient.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Tobacco streak virusTobacco streak virust0pathogene0
- Outcome
- virus showed three peakse0
- Value
- threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“purified virus showed three peaks in a frozen sucrose gradient”
e1“soybean (Glycine max)”
t0“Tobacco streak virus”
Claim · model-written summary
The soybean virus isolate was serologically related to TSV.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Tobacco streak virusTobacco streak virust0pathogene0
- Outcome
- serologically related to TSVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virus was serologically related to TSV”
e1“soybean (Glycine max)”
t0“Tobacco streak virus”
Claim · model-written summary
Electron microscopy detected spherical virus particles of 28 nm.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Tobacco streak virusTobacco streak virust0pathogene0
- Outcome
- spherical particlese0
- Value
- 28 nme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Electron microscopy detected 28 nm spherical particles”
e1“soybean (Glycine max)”
t0“Tobacco streak virus”
Claim · model-written summary
The viral coat protein had a reported Mr of 29.880 Da.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Tobacco streak virusTobacco streak virust0pathogene0
- Gene
- Coat protein (CP)e0
- Outcome
- Mre0
- Value
- 29.880 Dae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coat protein (CP) had a Mr of 29.880 Da”
e1“soybean (Glycine max)”
t0“Tobacco streak virus”
Claim · model-written summary
A 1028 nt viral fragment was amplified, cloned and sequenced.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Tobacco streak virusTobacco streak virust0pathogene0
- Outcome
- fragmente0
- Value
- 1028 nte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A fragment of 1028 nt was amplified, cloned and sequenced”
e1“soybean (Glycine max)”
t0“Tobacco streak virus”
Claim · model-written summary
One 717 nt open reading frame was associated with the viral coat protein.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Tobacco streak virusTobacco streak virust0pathogene0
- Gene
- CPe0
- Outcome
- open reading framee0
- Value
- 717 nte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One open reading frame with 717 nt was identified and associated to the CP”
e1“soybean (Glycine max)”
t0“Tobacco streak virus”
Claim · model-written summary
The coat protein gene had 83% identity with a TSV coat protein sequence from white clover.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Tobacco streak virusTobacco streak virust0pathogene0
- Gene
- CP genee0
- Outcome
- identitye0
- Value
- 83%e0
- Comparator
- TSV CP from white clover (Trifolium repens)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens)”
e1“soybean (Glycine max)”
t0“Tobacco streak virus”
Claim · model-written summary
The authors propose the soybean isolate as a TSV strain named TSV-BR.
- Taxon
- soybeanGlycine maxe1
- Interactor
- Tobacco streak virusTobacco streak virust0pathogene0
- Outcome
- strain of TSV named TSV-BRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this isolate be considered a strain of TSV named TSV-BR”
e1“soybean (Glycine max)”
t0“Tobacco streak virus”