Abstract
Tomato is one of the most economically and agriculturally important Solanaceous species and vegetable crops, serving as a model for examination of fruit biology and compound leaf development. Cytokinin is a plant hormone linked to the control of leaf development and is known to regulate a wide range of genes including many transcription factors. Currently there is little known of the leaf transcriptome in tomato and how it might be regulated by cytokinin. We employ high throughput mRNA sequencing technology and bioinformatic methodologies to robustly analyze cytokinin regulated tomato leaf transcriptomes. Leaf samples of two ages, 13d and 35d were treated with cytokinin or the solvent vehicle control dimethyl sulfoxide (DMSO) for 2 h or 24 h, after which RNA was extracted for sequencing. To confirm the accuracy of RNA sequencing results, we performed qPCR analysis of select transcripts identified as cytokinin regulated by the RNA sequencing approach. The resulting data provide the first hormone transcriptome analysis of leaves in tomato. Specifically we identified several previously untested tomato orthologs of cytokinin-related genes as well as numerous novel cytokinin-regulated transcripts in tomato leaves. Principal component analysis of the data indicates that length of cytokinin treatment and plant age are the major factors responsible for changes in transcripts observed in this study. Two hour cytokinin treatment showed a more robust transcript response indicated by both greater fold change of induced transcripts and the induction of twice as many cytokinin-related genes involved in signaling, metabolism, and transport in young vs. older leaves. This difference in transcriptome response in younger vs. older leaves was also found to a lesser extent with an extended (24 h) cytokinin treatment. Overall data presented here provides a solid foundation for future study of cytokinin and cytokinin regulated genes involved in compound leaf development or other developmental processes in tomato.
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Claims 7
Claim · model-written summary
The study provides the first hormone transcriptome analysis of tomato leaves.
- Taxon
- tomato
Solanum lycopersicume0 - Outcome
- hormone transcriptome analysise0
- Value
- firste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resulting data provide the first hormone transcriptome analysis of leaves in tomato.”
Claim · model-written summary
Previously untested tomato orthologs of cytokinin-related genes were identified.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- previously untested tomato orthologs of cytokinin-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified several previously untested tomato orthologs of cytokinin-related genes”
Claim · model-written summary
Numerous novel cytokinin-regulated transcripts were identified in tomato leaves.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- leavese0
- Outcome
- novel cytokinin-regulated transcriptse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“numerous novel cytokinin-regulated transcripts in tomato leaves”
Claim · model-written summary
Treatment length and plant age were the main factors associated with transcript changes.
- Taxon
- TomatoSolanum lycopersicumt0
- Organ / stage
- plant agee0
- Treatment
- cytokinin treatmente0
- Outcome
- changes in transcriptse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“length of cytokinin treatment and plant age are the major factors responsible for changes in transcripts observed in this study”
t0“Tomato Leaves”
Claim · model-written summary
After two hours of cytokinin treatment, induced transcripts showed greater fold change in young than older leaves.
- Taxon
- TomatoSolanum lycopersicumt0
- Organ / stage
- younge0
- Treatment
- Two hour cytokinin treatmente0
- Outcome
- fold change of induced transcriptse0
- Direction
- greatere0
- Comparator
- older leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two hour cytokinin treatment showed a more robust transcript response indicated by both greater fold change of induced transcripts and the induction of twice as many cytokinin-related genes involved in signaling, metabolism, and transport in young vs. older leaves”
t0“Tomato Leaves”
Claim · model-written summary
Two-hour cytokinin treatment induced twice as many cytokinin-related genes in young as older leaves.
- Taxon
- TomatoSolanum lycopersicumt0
- Organ / stage
- younge0
- Treatment
- Two hour cytokinin treatmente0
- Outcome
- cytokinin-related genes involved in signaling, metabolism, and transporte0
- Direction
- twice as manye0
- Value
- twice as manye0
- Comparator
- older leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two hour cytokinin treatment showed a more robust transcript response indicated by both greater fold change of induced transcripts and the induction of twice as many cytokinin-related genes involved in signaling, metabolism, and transport in young vs. older leaves”
t0“Tomato Leaves”
Claim · model-written summary
The transcriptome response remained stronger in younger than older leaves after 24 hours of cytokinin treatment, to a lesser extent.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- cytokinin treatmente0
- Outcome
- transcriptome responsee0
- Qualifier
- to a lesser extente0
- Comparator
- older leavese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This difference in transcriptome response in younger vs. older leaves was also found to a lesser extent with an extended (24 h) cytokinin treatment.”
t0“Tomato Leaves”