Abstract
The somatic chromosomes of Gossypium hirsutum, G. arboreum brown form, and G. arboreum white form were studied after staining with orcein, chromomycin A3 (CMA) and DAPI. Gossypium hirsutum possessed 2n=52 chromosomes with a gradual decrease in chromosome length. Both the forms of G. arboreum showed 2n=26 chromosomes with no such sharp decrease in chromosome length. The centromeric formula of G. hirsutum was 20 m+32 sm. The centromeric formulae of 10 m+16 sm and 14 m+12 sm were determined in the brown and white form of G. arboreum, respectively. Two big and 4 small CMA-positive bands were found in G. hirsutum. Four CMA-positive bands were found in G. arboreum brown form. The individual size of the band in the brown form was similar to that of big bands in G. hirsutum. Three CMA-positive bands were observed in the white form of G. arboreum which were smaller than those of the brown form. Gossypium hirsutum showed 3 DAPI-positive bands whereas no DAPI-positive band observed in brown and white form of G. arboreum. In G. hirsutum, 6 DAPI-negative bands appeared at the same location where CMA-positive bands were present. Three DAPI-negative bands were observed in brown and white form of G. arboreum at the same location where CMA-positive bands appeared. Reversible banding indicated the presence of GC-rich repeats. The banding pattern of G. hirsutum appeared to consider it as segmental allopolyploid. In spite of some similarities, the 2 forms of G. arboreum possessed unique fluorescent banding features.
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Claims 16
Claim · model-written summary
Gossypium hirsutum has 2n=52 chromosomes with a gradual decrease in chromosome length.
- Taxon
- Gossypium hirsutum
Gossypium hirsutume0 - Outcome
- chromosomes with a gradual decrease in chromosome lengthe0
- Value
- 2n=52e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gossypium hirsutum possessed 2n=52 chromosomes with a gradual decrease in chromosome length”
Claim · model-written summary
Both forms of G. arboreum have 2n=26 chromosomes without a sharp decrease in chromosome length.
- Taxon
- G. arboreum
Gossypium arboreume0 - Outcome
- chromosomes with no such sharp decrease in chromosome lengthe0
- Value
- 2n=26e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the forms of G. arboreum showed 2n=26 chromosomes with no such sharp decrease in chromosome length”
Claim · model-written summary
The centromeric formula of G. hirsutum was 20 m+32 sm.
- Taxon
- G. hirsutumGossypium hirsutume0
- Outcome
- centromeric formulae0
- Value
- 20 m+32 sme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The centromeric formula of G. hirsutum was 20 m+32 sm”
Claim · model-written summary
The brown form of G. arboreum had the centromeric formula 10 m+16 sm.
- Taxon
- G. arboreumGossypium arboreume0
- Material
- browne0
- Outcome
- centromeric formulaee0
- Value
- 10 m+16 sme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The centromeric formulae of 10 m+16 sm and 14 m+12 sm were determined in the brown and white form of G. arboreum, respectively”
Claim · model-written summary
The white form of G. arboreum had the centromeric formula 14 m+12 sm.
- Taxon
- G. arboreumGossypium arboreume0
- Material
- whitee0
- Outcome
- centromeric formulaee0
- Value
- 14 m+12 sme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The centromeric formulae of 10 m+16 sm and 14 m+12 sm were determined in the brown and white form of G. arboreum, respectively”
Claim · model-written summary
G. hirsutum showed two big and four small CMA-positive bands.
- Taxon
- G. hirsutumGossypium hirsutume0
- Outcome
- CMA-positive bandse0
- Value
- Two big and 4 smalle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two big and 4 small CMA-positive bands were found in G. hirsutum”
Claim · model-written summary
The brown form of G. arboreum showed four CMA-positive bands.
- Taxon
- G. arboreumGossypium arboreume0
- Material
- brown forme0
- Outcome
- CMA-positive bandse0
- Value
- Foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four CMA-positive bands were found in G. arboreum brown form”
Claim · model-written summary
The white form of G. arboreum showed three CMA-positive bands, smaller than those of the brown form.
- Taxon
- G. arboreumGossypium arboreume0
- Material
- white forme0
- Outcome
- CMA-positive bandse0
- Value
- Threee0
- Qualifier
- smaller than those of the brown forme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three CMA-positive bands were observed in the white form of G. arboreum which were smaller than those of the brown form”
Claim · model-written summary
The individual size of the CMA-positive band in the G. arboreum brown form was similar to that of the big bands in G. hirsutum.
- Taxon
- G. hirsutumGossypium hirsutume0
- Material
- brown forme0
- Outcome
- individual size of the bande0
- Qualifier
- similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The individual size of the band in the brown form was similar to that of big bands in G. hirsutum”
Claim · model-written summary
G. hirsutum showed three DAPI-positive bands.
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Outcome
- DAPI-positive bandse0
- Value
- 3 DAPI-positive bandse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gossypium hirsutum showed 3 DAPI-positive bands whereas no DAPI-positive band observed in brown and white form of G. arboreum”
Claim · model-written summary
No DAPI-positive band was observed in the brown and white forms of G. arboreum.
- Taxon
- G. arboreumGossypium arboreume0
- Material
- brown and white forme0
- Outcome
- no DAPI-positive band observede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gossypium hirsutum showed 3 DAPI-positive bands whereas no DAPI-positive band observed in brown and white form of G. arboreum”
Claim · model-written summary
In G. hirsutum six DAPI-negative bands appeared at the same locations as the CMA-positive bands.
- Taxon
- G. hirsutumGossypium hirsutume0
- Outcome
- DAPI-negative bandse0
- Value
- 6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In G. hirsutum, 6 DAPI-negative bands appeared at the same location where CMA-positive bands were present”
Claim · model-written summary
In the brown and white forms of G. arboreum three DAPI-negative bands appeared at the same locations as the CMA-positive bands.
- Taxon
- G. arboreumGossypium arboreume0
- Material
- brown and white forme0
- Outcome
- DAPI-negative bandse0
- Value
- Threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three DAPI-negative bands were observed in brown and white form of G. arboreum at the same location where CMA-positive bands appeared”
Claim · model-written summary
Reversible banding indicated the presence of GC-rich repeats.
- Outcome
- presence of GC-rich repeatse0
Not reportedTaxon
Verbatim
e0“Reversible banding indicated the presence of GC-rich repeats”
Claim · model-written summary
The banding pattern suggested that G. hirsutum is a segmental allopolyploid.
- Taxon
- G. hirsutumGossypium hirsutume0
- Outcome
- segmental allopolyploide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The banding pattern of G. hirsutum appeared to consider it as segmental allopolyploid”
Claim · model-written summary
The two forms of G. arboreum possessed unique fluorescent banding features despite some similarities.
- Taxon
- G. arboreumGossypium arboreume0
- Outcome
- unique fluorescent banding featurese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 2 forms of G. arboreum possessed unique fluorescent banding features”