Abstract
The goal of this study was to determine the expression profile of a late blight resistance gene, Rpi‐phu1 , after challenge with an incompatible Phytophthora infestans isolate. Relative expression levels were measured by quantitative real‐time PCR , before as well as 1, 3 and 5 days after contact with the pathogen. Plants tested included diploid and tetraploid breeding lines with the gene in different genetic backgrounds and plants of a chosen breeding line at different developmental stages. All tested potato breeding lines with the Rpi‐phu1 gene were highly resistant to P. infestans and so was the line tested at different developmental stages. No significant changes were detected in Rpi‐phu1 transcript level after pathogen challenge in any of the tested tetraploid potato lines. However, Rpi‐phu1 transcription in diploid lines was enhanced by contact with P. infestans . Basal expression of the Rpi‐phu1 gene was significantly lower in the youngest, 3‐week‐old plants than in 6‐week‐old plants. Nevertheless, 1 day after inoculation with the pathogen, transcript levels in these plants were slightly greater than in 6‐week‐old plants, indicating that plant age can influence transcription of the Rpi‐phu1 gene, but that this does not affect late blight resistance.
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Claims 6
Claim · model-written summary
All tested potato breeding lines carrying Rpi-phu1 were highly resistant to P. infestans.
- Taxon
- potato
Solanum tuberosume0 - Material
- potato breeding linese0
- Interactor
- P. infestans
Phytophthora infestanse0pathogen - Gene
- Rpi‐phu1e0
- Outcome
- highly resistant to P. infestanse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All tested potato breeding lines with the Rpi‐phu1 gene were highly resistant to P. infestans”
Claim · model-written summary
Resistance persisted across tested developmental stages.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- different developmental stagese0
- Outcome
- the line tested at different developmental stagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and so was the line tested at different developmental stages”
t0“potato”
Claim · model-written summary
Pathogen challenge did not significantly change Rpi-phu1 transcript levels in tetraploid lines.
- Taxon
- potatoSolanum tuberosume0
- Material
- tetraploid potato linese0
- Treatment
- pathogen challengee0
- Gene
- Rpi‐phu1e0
- Outcome
- Rpi‐phu1 transcript levele0
- Direction
- No significant changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No significant changes were detected in Rpi‐phu1 transcript level after pathogen challenge in any of the tested tetraploid potato lines”
Claim · model-written summary
Contact with P. infestans enhanced Rpi-phu1 transcription in diploid lines.
- Taxon
- potatoSolanum tuberosumt0
- Material
- diploid linese0
- Treatment
- contact with P. infestanse0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- Rpi‐phu1e0
- Outcome
- Rpi‐phu1 transcriptione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rpi‐phu1 transcription in diploid lines was enhanced by contact with P. infestans”
t0“potato”
Claim · model-written summary
Basal Rpi-phu1 expression was significantly lower in three-week-old than six-week-old plants.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- 3‐week‐old plantse0
- Gene
- Rpi‐phu1e0
- Outcome
- Basal expression of the Rpi‐phu1 genee0
- Direction
- lowere0
- Comparator
- 6‐week‐old plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Basal expression of the Rpi‐phu1 gene was significantly lower in the youngest, 3‐week‐old plants than in 6‐week‐old plants”
t0“potato”
Claim · model-written summary
One day after inoculation, young plants had slightly greater transcript levels than six-week-old plants.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- these plantse0
- Outcome
- transcript levelse0
- Direction
- slightly greatere0
- Qualifier
- 1 day after inoculation with the pathogene0
- Comparator
- 6‐week‐old plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1 day after inoculation with the pathogen, transcript levels in these plants were slightly greater than in 6‐week‐old plants”
t0“potato”
The same questions in other papers
infection · molecular: expression · Solanum tuberosum (potato)Positive and negative results
3 papers in the same direction as this paper · 17 in the opposite direction
- positive
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.