Abstract
Full-length cDNA copies of tobacco vein mottling virus (TVMV) RNA were constructed downstream from bacteriophage T7 or T3 RNA polymerase promoters. The plasmids were designed to produce in vitro transcripts containing, respectively, one or two guanosine residues at the 5' terminus not derived from the TVMV sequence and a single cytidine residue at the 3' terminus following the poly(A) tail. Introduction of transcripts from either plasmid into tobacco mesophyll protoplasts resulted in the accumulation of TVMV coat protein and RNA. Neither coat protein nor viral RNA accumulated in protoplasts inoculated with linearized cDNA or with in vitro transcripts synthesized in the absence of 7-methylguanosine(5')triphospho(5')guanosine (m7GpppG). Tobacco seedlings inoculated with native TVMV RNA developed symptoms a few days before those inoculated with in vitro transcripts; however, 3 weeks after inoculation, the symptoms produced by the two inocula were indistinguishable.
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Claims 5
Claim · model-written summary
Transcripts from either plasmid introduced into tobacco mesophyll protoplasts resulted in accumulation of TVMV coat protein and RNA.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- transcripts from either plasmide0
- Interactor
- TVMV
Tobacco vein mottling viruse0pathogen - Outcome
- accumulation of TVMV coat protein and RNAe0
- Comparator
- linearized cDNAe1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Introduction of transcripts from either plasmid into tobacco mesophyll protoplasts resulted in the accumulation of TVMV coat protein and RNA”
e1“Neither coat protein nor viral RNA accumulated in protoplasts inoculated with linearized cDNA or with in vitro transcripts synthesized in the absence of 7-methylguanosine(5')triphospho(5')guanosine (m7GpppG)”
Claim · model-written summary
Linearized cDNA did not yield accumulation of TVMV coat protein or viral RNA in tobacco protoplasts.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- linearized cDNAe1
- Interactor
- TVMVTobacco vein mottling viruse0pathogen
- Outcome
- coat protein nor viral RNA accumulatede1
- Comparator
- transcripts from either plasmide0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Introduction of transcripts from either plasmid into tobacco mesophyll protoplasts resulted in the accumulation of TVMV coat protein and RNA”
e1“Neither coat protein nor viral RNA accumulated in protoplasts inoculated with linearized cDNA or with in vitro transcripts synthesized in the absence of 7-methylguanosine(5')triphospho(5')guanosine (m7GpppG)”
Claim · model-written summary
In vitro transcripts synthesized without m7GpppG did not yield accumulation of TVMV coat protein or viral RNA in tobacco protoplasts.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- in vitro transcripts synthesized in the absence of 7-methylguanosine(5')triphospho(5')guanosine (m7GpppG)e1
- Interactor
- TVMVTobacco vein mottling viruse0pathogen
- Outcome
- coat protein nor viral RNA accumulatede1
- Comparator
- transcripts from either plasmide0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Introduction of transcripts from either plasmid into tobacco mesophyll protoplasts resulted in the accumulation of TVMV coat protein and RNA”
e1“Neither coat protein nor viral RNA accumulated in protoplasts inoculated with linearized cDNA or with in vitro transcripts synthesized in the absence of 7-methylguanosine(5')triphospho(5')guanosine (m7GpppG)”
Claim · model-written summary
Tobacco seedlings inoculated with native TVMV RNA developed symptoms a few days before those inoculated with in vitro transcripts.
- Taxon
- TobaccoNicotiana tabacume2
- Treatment
- native TVMV RNAe2
- Interactor
- TVMVTobacco vein mottling viruse2pathogen
- Outcome
- developed symptomse2
- Direction
- beforee2
- Value
- a few dayse2
- Comparator
- in vitro transcriptse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Tobacco seedlings inoculated with native TVMV RNA developed symptoms a few days before those inoculated with in vitro transcripts”
Claim · model-written summary
Three weeks after inoculation, tobacco seedlings inoculated with in vitro transcripts or native TVMV RNA showed indistinguishable symptoms.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- in vitro transcriptse2
- Interactor
- TVMVTobacco vein mottling viruse2pathogen
- Outcome
- symptomse3
- Qualifier
- 3 weeks after inoculatione3
- Comparator
- native TVMV RNAe2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Tobacco seedlings inoculated with native TVMV RNA developed symptoms a few days before those inoculated with in vitro transcripts”
e3“3 weeks after inoculation, the symptoms produced by the two inocula were indistinguishable”