Abstract
Monarda yellows occurring in southern Alberta was found to be associated with a phytoplasma. Using two pairs of universal primers, 16S ribosomal DNA fragments (about 1.5 and 1.2 kb) were amplified separately by polymerase chain reaction (PCR) from DNA samples that had been extracted from infected monarda. No such DNA bands were observed using DNA samples from uninfected monarda. The DNA fragment (1.2 kb) amplified by nested‐PCR was analysed and compared with western aster yellows (AY27, Canada), eastern aster yellows (EAY, USA), French hydrangea aster yellows (AYHF), Belgium hydrangea aster yellows (AYHB), clover proliferation (CP, Canada) and potato witches'‐broom (PWB, Canada) by means of restriction fragment length polymorphism (RFLP) using endonucleases Alu I, Mse I, Hpa II, Sau 3AI, Kpn I and Rsa I. The results showed that monarda yellows phytoplasma belongs to the aster yellows subclade and is different from CP and PWB. This is the first report of aster yellows phytoplasma infecting monarda.
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Claims 5
Claim · model-written summary
Monarda yellows in southern Alberta was associated with a phytoplasma.
MonardaNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Monarda yellows occurring in southern Alberta was found to be associated with a phytoplasma.”
Claim · model-written summary
Two 16S ribosomal DNA fragments were amplified from infected monarda, unlike uninfected monarda.
Not reportedInteractor
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“16S ribosomal DNA fragments (about 1.5 and 1.2 kb) were amplified separately by polymerase chain reaction (PCR) from DNA samples that had been extracted from infected monarda. No such DNA bands were observed using DNA samples from uninfected monarda.”
Claim · model-written summary
The monarda yellows phytoplasma belongs to the aster yellows subclade.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“monarda yellows phytoplasma belongs to the aster yellows subclade”
Claim · model-written summary
The monarda yellows phytoplasma differs from CP and PWB.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“monarda yellows phytoplasma belongs to the aster yellows subclade and is different from CP and PWB”
Claim · model-written summary
This is the first report of aster yellows phytoplasma infecting monarda.
- Interactor
- aster yellows phytoplasmaAster yellows phytoplasmae0pathogen
- Outcome
- infecting monardae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This is the first report of aster yellows phytoplasma infecting monarda.”