Abstract
Summary An investigation of root rot in A cacia mangium plantations in Indonesia generated over 1000 fungal isolates from field surveys at six locations. The majority of isolates were identified as G anoderma philippii or Ganoderma mastoporum by species‐specific PCR , but this still left 274 isolates to be identified. As barcoding initiatives for fungi are still in the early stages, to assist identification of these isolates in this way we developed a database of sequences based on identified sporocarps and also searched public DNA sequence databases for sequences with high similarity. The database of sequences from sporocarp collections is more limited than public DNA databases. This resulted in more reliance on public DNA databases for a high proportion of taxa. Several pathogenic species were identified, with P hellinus noxius occurring at several locations and on both A . mangium and E ucalyptus pellita . G anoderma steyaertanum was isolated from A . mangium roots and R igidoporus microporus from E . pellita roots, each at a single location only. Potential pitfalls of using public DNA databases for fungal identification are discussed and methods to avoid these and increase confidence in the identification are presented.
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Claims 8
Claim · model-written summary
Most root isolates from Acacia mangium were identified as Ganoderma philippii or Ganoderma mastoporum.
Verbatim
e0“An investigation of root rot in A cacia mangium plantations in Indonesia generated over 1000 fungal isolates from field surveys at six locations”
e1“The majority of isolates were identified as G anoderma philippii or G anoderma mastoporum”
Claim · model-written summary
Over 1000 fungal isolates were obtained from field surveys at six locations in Acacia mangium plantations.
Verbatim
e0“An investigation of root rot in A cacia mangium plantations in Indonesia generated over 1000 fungal isolates from field surveys at six locations”
Claim · model-written summary
274 isolates remained to be identified after species-specific PCR.
Verbatim
e0“An investigation of root rot in A cacia mangium plantations in Indonesia generated over 1000 fungal isolates from field surveys at six locations”
e2“but this still left 274 isolates to be identified”
Claim · model-written summary
Phellinus noxius occurs at several locations on Acacia mangium.
Verbatim
e0“An investigation of root rot in A cacia mangium plantations in Indonesia generated over 1000 fungal isolates from field surveys at six locations”
e3“with P hellinus noxius occurring at several locations and on both A . mangium and E ucalyptus pellita”
Claim · model-written summary
Phellinus noxius occurs at several locations on Eucalyptus pellita.
Verbatim
e3“with P hellinus noxius occurring at several locations and on both A . mangium and E ucalyptus pellita”
Claim · model-written summary
Ganoderma steyaertanum was isolated from Acacia mangium roots at a single location only.
Verbatim
e4“G anoderma steyaertanum was isolated from A . mangium roots and R igidoporus microporus from E . pellita roots, each at a single location only”
Claim · model-written summary
Rigidoporus microporus was isolated from Eucalyptus pellita roots at a single location only.
Verbatim
e4“G anoderma steyaertanum was isolated from A . mangium roots and R igidoporus microporus from E . pellita roots, each at a single location only”
Claim · model-written summary
The database of sequences from sporocarp collections is more limited than public DNA databases.
Verbatim
e0“An investigation of root rot in A cacia mangium plantations in Indonesia generated over 1000 fungal isolates from field surveys at six locations”
e5“The database of sequences from sporocarp collections is more limited than public DNA databases”