Abstract
Discrepancies of 2–20% were noted in nuclear DNA content estimates using mixed and unmixed extracts of several plant species combinations (target/standard). This may be because cytosolic components affect dye accessibility to DNA. Several dilution experiments showed clearly how cytosol concentration modified dye accessibility. Heat treatment suggested that dye accessibility could be related to chromatin sensitivity to decondensation. Some methods (nucleus isolation, dilution) can decrease, but not eliminate, stoichiometric error. The high sensitivity of the nucleus to the medium highlights the problem of reliability limits in estimating genome size. The choice between internal and external standardization and the interpretation of intraspecific variation in ‘nuclear DNA content’ are discussed.
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Claims 5
Claim · model-written summary
Mixed and unmixed extracts yielded nuclear DNA estimates differing by 2–20%.
Verbatim
e0“Discrepancies of 2–20% were noted in nuclear DNA content estimates using mixed and unmixed extracts of several plant species combinations (target/standard)”
Claim · model-written summary
Cytosol concentration modified dye accessibility in dilution experiments.
- Taxon
- PlantsViridiplantaet0
- Outcome
- dye accessibilitye0
- Qualifier
- cytosol concentratione0
Verbatim
e0“Several dilution experiments showed clearly how cytosol concentration modified dye accessibility”
t0“Plants”
Claim · model-written summary
Heat treatment suggested dye accessibility could relate to chromatin decondensation sensitivity.
- Taxon
- PlantsViridiplantaet0
- Treatment
- Heat treatmente0
- Outcome
- dye accessibilitye0
- Qualifier
- chromatin sensitivity to decondensatione0
Not reportedDirection
Verbatim
e0“Heat treatment suggested that dye accessibility could be related to chromatin sensitivity to decondensation”
t0“Plants”
Claim · model-written summary
Nucleus isolation can decrease but not eliminate stoichiometric error.
- Taxon
- PlantsViridiplantaet0
- Treatment
- nucleus isolatione0
- Outcome
- stoichiometric errore0
- Direction
- decreasee0
Not reportedComparator
Verbatim
e0“Some methods (nucleus isolation, dilution) can decrease, but not eliminate, stoichiometric error”
t0“Plants”
Claim · model-written summary
Dilution can decrease but not eliminate stoichiometric error.
- Taxon
- PlantsViridiplantaet0
- Treatment
- dilutione0
- Outcome
- stoichiometric errore0
- Direction
- decreasee0
Not reportedComparator
Verbatim
e0“Some methods (nucleus isolation, dilution) can decrease, but not eliminate, stoichiometric error”
t0“Plants”