Abstract
Tetraplold alfalfa ( Medicago sativa L.) germplasm, in which two‐thirds of the plants are capable of regeneration from callus tissue in culture, has been developed by recurrent selection. It descends from one ‘DuPuits’ and four ‘Saranac’ clones which regenerated in the cycle 0 population. Frequency of regeneration was 12% in cycle 0, about 50% in cycle 1, and 67% in cycle 2. Seed produced by hand‐intercrossing 75 plants regenerated from cycle 2 has been termed ‘Regen‐S’ (Regen for regeneration; S for Saranac). It is purple‐flowered and phenotypically similar to Saranac. One‐gram lots of Regen‐S seed are presently available for distribution, and larger amounts are expected to be available in late 1975. A yellow‐flowered regenerator ‘Regen‐Y,’ is now being bred from selections out of ‘Rhizoma,’ ‘Rambler,’ and ‘Drylander.’ Small amounts of Regen‐Y seed are also presently available, thus providing a contrasting qualitative genetic marker stock.
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Claims 4
Claim · model-written summary
Frequency of callus regeneration in alfalfa rose from 12% in cycle 0 to about 50% in cycle 1 and 67% in cycle 2.
- Taxon
- Medicago sativa
Medicago sativae0 - Outcome
- Frequency of regeneratione1
- Value
- 12% in cycle 0, about 50% in cycle 1, and 67% in cycle 2e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tetraplold alfalfa ( Medicago sativa L.) germplasm”
e1“Frequency of regeneration was 12% in cycle 0, about 50% in cycle 1, and 67% in cycle 2”
Claim · model-written summary
Germplasm developed by recurrent selection has two-thirds of plants capable of regeneration from callus tissue in culture.
- Taxon
- Medicago sativaMedicago sativae0
- Material
- DuPuitse3
- Treatment
- recurrent selectione2
- Outcome
- regeneration from callus tissue in culturee2
- Value
- two‐thirdse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tetraplold alfalfa ( Medicago sativa L.) germplasm”
e2“in which two‐thirds of the plants are capable of regeneration from callus tissue in culture, has been developed by recurrent selection”
e3“It descends from one ‘DuPuits’ and four ‘Saranac’ clones which regenerated in the cycle 0 population”
Claim · model-written summary
Regen-S seed is purple-flowered and phenotypically similar to Saranac.
- Taxon
- Medicago sativaMedicago sativae0
- Outcome
- phenotypically similare4
- Qualifier
- purple‐flowerede4
- Comparator
- Saranace4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tetraplold alfalfa ( Medicago sativa L.) germplasm”
e4“It is purple‐flowered and phenotypically similar to Saranac”
Claim · model-written summary
The yellow-flowered regenerator Regen-Y provides a contrasting qualitative genetic marker stock.
- Taxon
- alfalfaMedicago sativae0
- Outcome
- qualitative genetic marker stocke5
- Value
- Small amountse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tetraplold alfalfa ( Medicago sativa L.) germplasm”
e5“Small amounts of Regen‐Y seed are also presently available, thus providing a contrasting qualitative genetic marker stock”