Abstract
Maize seeds were inoculated with a commercial inoculant containing 1.3 × 10 7 Azospirillum lipoferum CRT1 cells. After 24 or 48 h, bacteria were washed from the seed surface. Washed and unwashed seeds were then planted in pots containing perlite and grown for 28 days under greenhouse conditions. Whatever the density of Azospirillum at planting, the number of these bacteria at the end of the experiment was similar (1.9-8.0 × 10 7 bacteria·plant -1 ). However, comparison of root surface areas of the plants were different depending on the period of contact between seeds and the density of the inoculum. Twenty-four hours of contact was not sufficient to increase root growth surface areas. Contact for 48 h permitted us to obtain root surface areas comparable with those measured after a continuous contact. These results showed that in order to promote maize root surface areas, an optimal density of Azospirillum is not required during the whole cultural cycle. This optimal density is indispensable only up to the emergence of the radicle.Key words: Azospirillum, maize, inoculation, PGPR.
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Claims 5
Claim · model-written summary
Final Azospirillum abundance was similar regardless of its density at planting, at 1.9-8.0 × 10 7 bacteria·plant -1.
- Taxon
- Azospirillum
Azospirillume0 - Outcome
- number of these bacteriae0
- Direction
- similare0
- Value
- 1.9-8.0 × 10 7 bacteria·plant -1e0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Whatever the density of Azospirillum at planting, the number of these bacteria at the end of the experiment was similar (1.9-8.0 × 10 7 bacteria·plant -1 )”
t0“maize”
Claim · model-written summary
Maize root surface areas differed with seed contact duration and inoculum density.
- Treatment
- period of contact between seeds and the density of the inoculume0
- Interactor
- Azospirillum lipoferum CRT1Azospirillum lipoferumt0symbiont
- Outcome
- root surface arease0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“comparison of root surface areas of the plants were different depending on the period of contact between seeds and the density of the inoculum”
t0“Azospirillum lipoferum CRT1 during maize seed germination”
Claim · model-written summary
Twenty-four hours of inoculant contact was insufficient to increase maize root surface area.
- Treatment
- Twenty-four hours of contacte0
- Interactor
- Azospirillum lipoferum CRT1Azospirillum lipoferumt0symbiont
- Outcome
- root growth surface arease0
- Direction
- not sufficient to increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twenty-four hours of contact was not sufficient to increase root growth surface areas.”
t0“Azospirillum lipoferum CRT1 during maize seed germination”
Claim · model-written summary
Forty-eight hours of inoculant contact produced maize root surface areas comparable to continuous contact.
- Treatment
- Contact for 48 he0
- Interactor
- Azospirillum lipoferum CRT1Azospirillum lipoferumt0symbiont
- Outcome
- root surface arease0
- Direction
- comparablee0
- Comparator
- continuous contacte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Contact for 48 h permitted us to obtain root surface areas comparable with those measured after a continuous contact.”
t0“Azospirillum lipoferum CRT1 during maize seed germination”
Claim · model-written summary
An optimal Azospirillum density is required only until radicle emergence to promote maize root surface area, rather than throughout cultivation.
- Interactor
- AzospirillumAzospirillume0symbiont
- Outcome
- optimal densitye0
- Qualifier
- only up to the emergence of the radiclee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results showed that in order to promote maize root surface areas, an optimal density of Azospirillum is not required during the whole cultural cycle. This optimal density is indispensable only up to the emergence of the radicle.”