Abstract
Two cDNAs (AtPT1 and AtPT2) encoding plant phosphate transporters have been isolated from a library prepared with mRNA extracted from phosphate-starved Arabidopsis thaliana roots, The encoded polypeptides are 78% identical to each other and show high degree of amino acid sequence similarity with high-affinity phosphate transporters of Saccharomyces cerevisiae, Neurospora crassa, and the mycorrhizal fungus Glomus versiforme. The AtPT1 and AtPT2 polypeptides are integral membrane proteins predicted to contain 12 membrane-spanning domains separated into two groups of six by a large charged hydrophilic region. Upon expression, both AtPT1 and AtPT2 were able to complement the pho84 mutant phenotype of yeast strain NS219 lacking the high-affinity phosphate transport activity. AtPT1 and AtPT2 are representatives of two distinct, small gene families in A. thaliana. The transcripts of both genes are expressed in roots and are not detectable in leaves. The steady-state level of their mRNAs increases in response to phosphate starvation.
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Claims 8
Claim · model-written summary
Two Arabidopsis phosphate transporter cDNAs, AtPT1 and AtPT2, were isolated.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Organ / stage
- rootse0
- Gene
- AtPT1 and AtPT2e0
- Outcome
- Two cDNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two cDNAs (AtPT1 and AtPT2) encoding plant phosphate transporters have been isolated from a library prepared with mRNA extracted from phosphate-starved Arabidopsis thaliana roots”
Claim · model-written summary
The two encoded polypeptides are 78% identical.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- 78% identicale0
- Value
- 78%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The encoded polypeptides are 78% identical to each other”
t0“Phosphate transporters from the higher plant Arabidopsis thaliana.”
Claim · model-written summary
The encoded polypeptides show high similarity to fungal phosphate transporters.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- high degree of amino acid sequence similaritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“show high degree of amino acid sequence similarity with high-affinity phosphate transporters of Saccharomyces cerevisiae, Neurospora crassa, and the mycorrhizal fungus Glomus versiforme”
t0“Phosphate transporters from the higher plant Arabidopsis thaliana.”
Claim · model-written summary
AtPT1 and AtPT2 are predicted integral membrane proteins with 12 membrane-spanning domains.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AtPT1 and AtPT2e0
- Outcome
- 12 membrane-spanning domainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The AtPT1 and AtPT2 polypeptides are integral membrane proteins predicted to contain 12 membrane-spanning domains separated into two groups of six by a large charged hydrophilic region”
t0“Phosphate transporters from the higher plant Arabidopsis thaliana.”
Claim · model-written summary
Both proteins complemented the yeast pho84 mutant phenotype.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- expressione0
- Gene
- AtPT1 and AtPT2e0
- Outcome
- complement the pho84 mutant phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon expression, both AtPT1 and AtPT2 were able to complement the pho84 mutant phenotype of yeast strain NS219 lacking the high-affinity phosphate transport activity”
t0“Phosphate transporters from the higher plant Arabidopsis thaliana.”
Claim · model-written summary
AtPT1 and AtPT2 represent distinct small gene families in Arabidopsis.
- Taxon
- A. thalianaArabidopsis thalianae0
- Gene
- AtPT1 and AtPT2e0
- Outcome
- two distinct, small gene familiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtPT1 and AtPT2 are representatives of two distinct, small gene families in A. thaliana”
Claim · model-written summary
Both transcripts are expressed in roots and undetectable in leaves.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- rootse0
- Outcome
- expressed in roots and are not detectable in leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcripts of both genes are expressed in roots and are not detectable in leaves”
t0“Phosphate transporters from the higher plant Arabidopsis thaliana.”
Claim · model-written summary
Phosphate starvation increases steady-state levels of both mRNAs.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- phosphate starvatione0
- Outcome
- steady-state level of their mRNAse0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The steady-state level of their mRNAs increases in response to phosphate starvation”
t0“Phosphate transporters from the higher plant Arabidopsis thaliana.”
The same questions in other papers
phosphorus · molecular: metabolites · Arabidopsis thaliana (thale cress)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- negative
- no effect
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.