Paper · 2013 · Journal of Phytopathology

The Complete Nucleotide Sequence of the RNA 1 of a Chinese Isolate of Tomato chlorosis virus

Miriam Karwitha; Zhike Feng; Min Yao; et al.

3
claims
6
verbatim quotes
5
citations
3
research areas
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Open access

Abstract

Abstract Interveinal leaf chlorosis, brittleness, limited necrotic flecking or bronzing developed on greenhouse‐grown tobacco and tomato plants at N anjing A gricultural U niversity from 2010 to 2013. A positive RT ‐ PCR using a pair of degenerate primers for C rinivirus confirmed the diseased plants were infected with T omato chlorosis virus ( T o CV ). The complete RNA 1 genomic sequence of this T o CV isolate was determined; it comprises of 8596 nucleotides with four open reading frames. Phylogenetic analysis of T o CV isolates from diverse geographical regions categorized the T o CV isolates into two main groups. Group one consisted of C hinese, A merican‐ F lorida, G reek and B razilian isolates, while G roup two contained only the S panish isolate. The first group had two subgroups, one of C hinese and A merican‐ F lorida isolates, while the other subgroup had G reek and B razilian isolates. This is the first study of the complete nucleotide sequence of the RNA 1 of To CV isolated from C hina.

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Claims 3

Observationobservedoriginal resultmeasured

Claim · model-written summary

The complete RNA 1 of a Chinese Tomato chlorosis virus isolate is 8596 nucleotides long with four open reading frames.

Material
T o CV isolatee1
Interactor
T o CVe1pathogen
Outcome
complete RNA 1 genomic sequencee1
Value
8596 nucleotidese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“tobacco and tomato plants”

✓ located verbatim in the source · abstract, characters 118–143 · show in text ↑
e1

“The complete RNA 1 genomic sequence of this T o CV isolate was determined; it comprises of 8596 nucleotides with four open reading frames”

✓ located verbatim in the source · abstract, characters 357–494 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Tomato chlorosis virus isolates fell into two main phylogenetic groups.

Interactor
T o CVe1pathogen
Outcome
categorized the T o CV isolates into two main groupse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“tobacco and tomato plants”

✓ located verbatim in the source · abstract, characters 118–143 · show in text ↑
e1

“Phylogenetic analysis of T o CV isolates from diverse geographical regions categorized the T o CV isolates into two main groups”

✓ located verbatim in the source · abstract, characters 496–623 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Tobacco and tomato plants developed interveinal leaf chlorosis under greenhouse conditions.

Outcome
Interveinal leaf chlorosise3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“tobacco and tomato plants”

✓ located verbatim in the source · abstract, characters 118–143 · show in text ↑
e3

“Interveinal leaf chlorosis”

✓ located verbatim in the source · abstract, characters 9–35 · show in text ↑