Abstract
AbstractA method for the dry-preservation of fixed plant material, root tips and buds, is described. The method seems to be advantageous on long expeditions and when material has to be sent away.The material is transferred from the fixative to 70% alcohol (3 changes, 1/2 hour in the last). It is dried on blotting-paper. The dried material may be preserved a long time. Material kept dry for 4 1/2 years has proved to be quite satisfactory. Drying has been tried after fixation with CRAF-solutions (Webber and Randolph modifications) and fixatives containing osmic acid (Fleming-Benda and 2BD).The dry material is swollen by keeping for 2 days in 10% alcohol. It is embedded in paraffin according to the usual method. A satisfactory staining has been obtained after these fixatives using iodine-gentian-violet and Feulgen stainings. In addition to chromosome counts dry material may be used for chromosome morphology studies.Dried material fixed in aceto-alcohol (1:3) has not turned out to be specially suitable for squash preparations owing to the fragility of the chromosomes. If strong pressure is not applied, satisfactory results may, however, be obtained.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Dry-preserved fixed plant material remained quite satisfactory after 4 1/2 years of storage.
Verbatim
e1“Material kept dry for 4 1/2 years has proved to be quite satisfactory”
e2“A method for the dry-preservation of fixed plant material, root tips and buds, is described”
t0“The Dry Preservation of Fixed Plant Material”
Claim · model-written summary
Staining of dry-preserved fixed plant material with iodine-gentian-violet and Feulgen stains is satisfactory.
Verbatim
e3“A satisfactory staining has been obtained after these fixatives using iodine-gentian-violet and Feulgen stainings”
t0“The Dry Preservation of Fixed Plant Material”
Claim · model-written summary
Dry material fixed in aceto-alcohol (1:3) is not specially suitable for squash preparations because the chromosomes are fragile.
Verbatim
e4“Dried material fixed in aceto-alcohol (1:3) has not turned out to be specially suitable for squash preparations owing to the fragility of the chromosomes”
t0“The Dry Preservation of Fixed Plant Material”
Claim · model-written summary
Without strong pressure, satisfactory squash results may be obtained from dry-preserved plant material.
Verbatim
e5“If strong pressure is not applied, satisfactory results may, however, be obtained”
t0“The Dry Preservation of Fixed Plant Material”
Claim · model-written summary
Dry-preserved plant material may be used for chromosome morphology studies in addition to chromosome counts.
Verbatim
e6“In addition to chromosome counts dry material may be used for chromosome morphology studies”
t0“The Dry Preservation of Fixed Plant Material”
Claim · model-written summary
The dry preservation method is advantageous on long expeditions and when material has to be sent away.
Verbatim
e7“The method seems to be advantageous on long expeditions and when material has to be sent away”
t0“The Dry Preservation of Fixed Plant Material”