Paper · 1998 · Phytopathology

Protein(s) from the Gram-Positive Bacterium Clavibacter michiganensis subsp. michiganensis Induces a Hypersensitive Response in Plants

C. Alarcón; J. Castro; F. Muñoz; et al.

6
claims
8
verbatim quotes
26
citations
1
research areas
—
Not open access

Abstract

ABSTRACT The gram-positive tomato pathogen Clavibacter michiganensis subsp. michiganensis induced a local necrotic response on four-o'clock (Mirabilis jalapa) and tobacco (Nicotiana tabacum) plants. This necrosis response was characteristic of the hypersensitive response (HR). The cell-free culture supernatant from strain CMM623 also induced a necrosis that was phenotypically similar to that induced by the bacteria. Inhibitors of plant metabolism suppressed the necrotic reaction of both M. jalapa and tobacco. The HR-inducing activity present in the supernatant was heat stable, sensitive to proteases, and had an apparent molecular mass in the range of 35 to 50 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The properties observed for the necrosis-inducing activity resembled harpin and PopA described from gram-negative phytopathogenic bacteria.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Clavibacter induced local necrosis on four-o'clock plants.

Taxon
four-o'clock (Mirabilis jalapa)Mirabilis jalapae0
Interactor
Clavibacter michiganensis subsp. michiganensisClavibacter michiganensis subsp. michiganensise0pathogen
Outcome
local necrotic responsee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mirabilis jalapa
Mirabilis jalapa · TaxonWildfeuer · CC BY 2.5 · Wikimedia Commons ↗
Clavibacter michiganensis subsp. michiganensis
Clavibacter michiganensis subsp. michiganensis · Interactor · picture of the speciesMary Ann Hansen, Virginia Polytechnic Institute and State University, Bugwood.org · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The gram-positive tomato pathogen Clavibacter michiganensis subsp. michiganensis induced a local necrotic response on four-o'clock (Mirabilis jalapa) and tobacco (Nicotiana tabacum) plants”

✓ located verbatim in the source · abstract, characters 9–197 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Clavibacter induced local necrosis on tobacco plants.

Taxon
tobacco (Nicotiana tabacum)Nicotiana tabacume0
Interactor
Clavibacter michiganensis subsp. michiganensisClavibacter michiganensis subsp. michiganensise0pathogen
Outcome
local necrotic responsee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗
Clavibacter michiganensis subsp. michiganensis
Clavibacter michiganensis subsp. michiganensis · Interactor · picture of the speciesMary Ann Hansen, Virginia Polytechnic Institute and State University, Bugwood.org · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The gram-positive tomato pathogen Clavibacter michiganensis subsp. michiganensis induced a local necrotic response on four-o'clock (Mirabilis jalapa) and tobacco (Nicotiana tabacum) plants”

✓ located verbatim in the source · abstract, characters 9–197 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

CMM623 culture supernatant induced phenotypically similar necrosis.

Taxon
tobacco (Nicotiana tabacum)Nicotiana tabacume1
Treatment
cell-free culture supernatant from strain CMM623e0
Outcome
necrosise0
Direction
inducede0
Comparator
the bacteriae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The cell-free culture supernatant from strain CMM623 also induced a necrosis that was phenotypically similar to that induced by the bacteria”

✓ located verbatim in the source · abstract, characters 278–418 · show in text ↑
e1

“tobacco (Nicotiana tabacum)”

✓ located verbatim in the source · abstract, characters 163–190 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Plant metabolism inhibitors suppressed necrosis in M. jalapa.

Taxon
M. jalapaMirabilis jalapae0
Treatment
Inhibitors of plant metabolisme0
Outcome
necrotic reactione0
Direction
suppressede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mirabilis jalapa
Mirabilis jalapa · TaxonWildfeuer · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Inhibitors of plant metabolism suppressed the necrotic reaction of both M. jalapa and tobacco”

✓ located verbatim in the source · abstract, characters 420–513 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Plant metabolism inhibitors suppressed necrosis in tobacco.

Treatment
Inhibitors of plant metabolisme0
Outcome
necrotic reactione0
Direction
suppressede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Inhibitors of plant metabolism suppressed the necrotic reaction of both M. jalapa and tobacco”

✓ located verbatim in the source · abstract, characters 420–513 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The supernatant HR-inducing activity was heat stable and protease sensitive, with apparent mass 35 to 50 kDa.

Taxon
tobacco (Nicotiana tabacum)Nicotiana tabacume1
Outcome
HR-inducing activitye0
Value
35 to 50 kDae0
Qualifier
heat stable, sensitive to proteasese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The HR-inducing activity present in the supernatant was heat stable, sensitive to proteases, and had an apparent molecular mass in the range of 35 to 50 kDa”

✓ located verbatim in the source · abstract, characters 515–671 · show in text ↑
e1

“tobacco (Nicotiana tabacum)”

✓ located verbatim in the source · abstract, characters 163–190 · show in text ↑