Abstract
ABSTRACT The gram-positive tomato pathogen Clavibacter michiganensis subsp. michiganensis induced a local necrotic response on four-o'clock (Mirabilis jalapa) and tobacco (Nicotiana tabacum) plants. This necrosis response was characteristic of the hypersensitive response (HR). The cell-free culture supernatant from strain CMM623 also induced a necrosis that was phenotypically similar to that induced by the bacteria. Inhibitors of plant metabolism suppressed the necrotic reaction of both M. jalapa and tobacco. The HR-inducing activity present in the supernatant was heat stable, sensitive to proteases, and had an apparent molecular mass in the range of 35 to 50 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The properties observed for the necrosis-inducing activity resembled harpin and PopA described from gram-negative phytopathogenic bacteria.
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Claims 6
Claim · model-written summary
Clavibacter induced local necrosis on four-o'clock plants.
- Taxon
- four-o'clock (Mirabilis jalapa)
Mirabilis jalapae0 - Interactor
- Clavibacter michiganensis subsp. michiganensis
Clavibacter michiganensis subsp. michiganensise0pathogen - Outcome
- local necrotic responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.


Verbatim
e0“The gram-positive tomato pathogen Clavibacter michiganensis subsp. michiganensis induced a local necrotic response on four-o'clock (Mirabilis jalapa) and tobacco (Nicotiana tabacum) plants”
Claim · model-written summary
Clavibacter induced local necrosis on tobacco plants.
- Taxon
- tobacco (Nicotiana tabacum)
Nicotiana tabacume0
- Interactor
- Clavibacter michiganensis subsp. michiganensis
Clavibacter michiganensis subsp. michiganensise0pathogen - Outcome
- local necrotic responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The gram-positive tomato pathogen Clavibacter michiganensis subsp. michiganensis induced a local necrotic response on four-o'clock (Mirabilis jalapa) and tobacco (Nicotiana tabacum) plants”
Claim · model-written summary
CMM623 culture supernatant induced phenotypically similar necrosis.
- Taxon
- tobacco (Nicotiana tabacum)
Nicotiana tabacume1
- Treatment
- cell-free culture supernatant from strain CMM623e0
- Outcome
- necrosise0
- Direction
- inducede0
- Comparator
- the bacteriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cell-free culture supernatant from strain CMM623 also induced a necrosis that was phenotypically similar to that induced by the bacteria”
e1“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Plant metabolism inhibitors suppressed necrosis in M. jalapa.
- Taxon
- M. jalapa
Mirabilis jalapae0 - Treatment
- Inhibitors of plant metabolisme0
- Outcome
- necrotic reactione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Inhibitors of plant metabolism suppressed the necrotic reaction of both M. jalapa and tobacco”
Claim · model-written summary
Plant metabolism inhibitors suppressed necrosis in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0
- Treatment
- Inhibitors of plant metabolisme0
- Outcome
- necrotic reactione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibitors of plant metabolism suppressed the necrotic reaction of both M. jalapa and tobacco”
Claim · model-written summary
The supernatant HR-inducing activity was heat stable and protease sensitive, with apparent mass 35 to 50 kDa.
- Taxon
- tobacco (Nicotiana tabacum)
Nicotiana tabacume1
- Outcome
- HR-inducing activitye0
- Value
- 35 to 50 kDae0
- Qualifier
- heat stable, sensitive to proteasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The HR-inducing activity present in the supernatant was heat stable, sensitive to proteases, and had an apparent molecular mass in the range of 35 to 50 kDa”
e1“tobacco (Nicotiana tabacum)”