Abstract
In plants, synthesis of the iron-storage protein ferritin in response to iron is not regulated at the translational level; this is in contrast to ferritin synthesis in animals. Part of the response is mediated through a transduction pathway which involves the plant hormone abscisic acid. In this work, we report the cloning and sequencing of two maize ferritin genes (ZmFer1 and ZmFer2) coding for members of the two ferritin mRNA subclasses, FM1 and FM2, respectively. Although plant and animal ferritins are closely related proteins, a major difference is observed between the organisation of the genes. Both maize ferritin genes are organised as eight exons and seven introns, the positions of which are identical within the two genes, while animal ferritin genes are interrupted by three introns, at positions different from those found in maize genes. Sequence divergence between the 3' untranslated regions of these genes has allowed the use of specific probes to study the accumulation of FM1 and FM2 transcripts in response to various environmental cues. Such probes have shown that FM1 and FM2 transcripts accumulate with differential kinetics in response to iron; FM1 mRNA accumulate earlier than FM2 mRNA and only FM2 transcripts accumulate in response to exogenous abscisic acid or water stress. Mapping of the transcriptional initiation region of these two genes defined their 5' upstream regions and allowed a sequence comparison of their promoters, which appeared highly divergent. This raises the possibility that the differential accumulation of FM1 and FM2 mRNAs in response to iron, abscisic acid and drought could be due to differential transcription of ZmFer1 and ZmFer2.
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Claims 9
Claim · model-written summary
ZmFer1 was cloned and sequenced as a gene coding for the FM1 ferritin mRNA subclass.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Ferritin Genes”
e0“cloning and sequencing of two maize ferritin genes (ZmFer1 and ZmFer2) coding for members of the two ferritin mRNA subclasses, FM1 and FM2, respectively”
Claim · model-written summary
ZmFer2 was cloned and sequenced as a gene coding for the FM2 ferritin mRNA subclass.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Ferritin Genes”
e0“cloning and sequencing of two maize ferritin genes (ZmFer1 and ZmFer2) coding for members of the two ferritin mRNA subclasses, FM1 and FM2, respectively”
Claim · model-written summary
Both maize ferritin genes have eight exons and seven introns at identical positions.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both maize ferritin genes are organised as eight exons and seven introns, the positions of which are identical within the two genes”
Claim · model-written summary
Maize ferritin gene intron organization differs from animal ferritin genes, which have three introns at different positions.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while animal ferritin genes are interrupted by three introns, at positions different from those found in maize genes”
Claim · model-written summary
FM1 mRNA accumulated earlier than FM2 mRNA in response to iron.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Ferritin Genes”
e0“FM1 and FM2 transcripts accumulate with differential kinetics in response to iron; FM1 mRNA accumulate earlier than FM2 mRNA”
Claim · model-written summary
Only FM2 transcripts accumulated in response to exogenous abscisic acid.
- Treatment
- exogenous abscisic acide0
- Gene
- FM2 transcriptse0
- Outcome
- FM2 transcripts accumulatee0
- Direction
- accumulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Ferritin Genes”
e0“only FM2 transcripts accumulate in response to exogenous abscisic acid or water stress”
Claim · model-written summary
Only FM2 transcripts accumulated in response to water stress.
- Treatment
- water stresse0
- Gene
- FM2 transcriptse0
- Outcome
- FM2 transcripts accumulatee0
- Direction
- accumulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Ferritin Genes”
e0“only FM2 transcripts accumulate in response to exogenous abscisic acid or water stress”
Claim · model-written summary
The ZmFer1 and ZmFer2 promoters appeared highly divergent.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoters, which appeared highly divergent”
e1“differential transcription of ZmFer1 and ZmFer2”
t0“Maize Ferritin Genes”
Claim · model-written summary
The transcriptional initiation regions of ZmFer1 and ZmFer2 were mapped and their upstream regions defined.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mapping of the transcriptional initiation region of these two genes defined their 5' upstream regions”
e1“differential transcription of ZmFer1 and ZmFer2”
t0“Maize Ferritin Genes”
The same questions in other papers
drought · molecular: expression · Zea mays (maize)Positive and negative results
42 papers in the same direction as this paper · 22 in the opposite direction
- no effect
- negative
- no effect
61 papers more in this group
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