Abstract
When preparing winter flower-bud material for microscopic examination, unbroken series of sections cannot be obtained by the ordinary methods of dehydrating, imbedding, etc. This is due to the diverse but characteristic structures found in a resting bud. These structures include heavy impervious protective scales, dense mats of hairs between the young leaves, flowers and bracts and a delicate embryonic tissue at the tip. A continuous series of sections may be obtained by (1) making use of extremely sharp tungsten needles to remove the more minute scales and bracts, especially those between the embryonic flowers; (2) soaking the material for at least two months m 70% alcohol; (3) using n-butyl alcohol instead of absolute alcohol and xylol; (4) keeping the material continuously at low pressures; and (5) using an alcoholic stain. Each of these additional steps helps, but all are necessary for completely satisfactory results. A method by which the special tungsten needles may be made is described. The continuous treatment at low pressures is made possible by using a two ounce bottle fitted with a capillary tube and stopcock The stains which proved most satisfactory were alcoholic solutions of safranin and acid fuchsin.
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Claims 4
Claim · model-written summary
Ordinary dehydration and embedding methods failed to yield unbroken winter bud sections.
- Outcome
- unbroken series of sectionse0
- Direction
- cannot be obtainede0
- Comparator
- ordinary methods of dehydrating, imbeddinge0
Not reportedTaxon
Verbatim
e0“unbroken series of sections cannot be obtained by the ordinary methods of dehydrating, imbedding”
Claim · model-written summary
The combined needle, soaking, solvent, pressure and staining steps produced continuous sections.
- Outcome
- continuous series of sectionse0
- Direction
- may be obtainede0
- Comparator
- absolute alcohol and xylole0
Not reportedTaxon
Verbatim
e0“A continuous series of sections may be obtained by (1) making use of extremely sharp tungsten needles to remove the more minute scales and bracts, especially those between the embryonic flowers; (2) soaking the material for at least two months m 70% alcohol; (3) using n-butyl alcohol instead of absolute alcohol and xylol; (4) keeping the material continuously at low pressures; and (5) using an alcoholic stain”
Claim · model-written summary
Each added step helped, but all were necessary for fully satisfactory results.
Not reportedTaxon
Verbatim
e0“Each of these additional steps helps, but all are necessary for completely satisfactory results”
Claim · model-written summary
Alcoholic safranin and acid fuchsin stains were most satisfactory.
- Outcome
- most satisfactorye0
- Direction
- most satisfactorye0
- Comparator
- alcoholic solutions of safranin and acid fuchsine0
Not reportedTaxon
Verbatim
e0“The stains which proved most satisfactory were alcoholic solutions of safranin and acid fuchsin”