Abstract
ABSTRACT The fungus Thecaphora solani (syn.: Angiosorus solani), the causal agent of potato smut, was cultivated in vitro for the first time. Teliospores obtained from galls of infected potato plants were used to inoculate commonly used solid and liquid media. The teliospores produced two kinds of vegetative tissue depending on the nutrient status of the media. A very slow radial-growing, hyaline, and septate mycelium, as usually seen in most of the in vitro-cultivated filamentous fungi, was obtained in wateragar medium after 30 to 40 days. On the other hand, a white, sponge-like mycelial mass was obtained in HCM + 1% activated charcoal, and on common potato dextrose agar or malt-yeast-peptone solid or liquid media, after 40 to 50 days under lab conditions. The identity among teliospores and the sponge-like mycelial mass was corroborated by DNA fingerprinting and partial sequencing of the large subunit (LSU) rDNA region. The sexual cycle of the pathogen was completed under lab conditions based on the development of teliospores on the sponge-like mycelial mass. The first attempt to reproduce the disease under controlled conditions was successful, inducing a gall in a cv. Desirée potato explant cultivated in vitro inoculated with radial-growing mycelia. Phylogenetic analysis of LSU rDNA data of the genus Thecaphora and other smut fungi confirmed the initial classification of the pathogen as T. solani.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
The potato smut fungus Thecaphora solani was cultivated in vitro for the first time.
- Taxon
- Thecaphora solani
Thecaphora solanie1 - Outcome
- cultivated in vitro for the first timee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The fungus Thecaphora solani (syn.: Angiosorus solani), the causal agent of potato smut, was cultivated in vitro for the first time”
Claim · model-written summary
Teliospores produced a very slow radial-growing, hyaline, septate mycelium in water agar medium after 30 to 40 days.
- Taxon
- Thecaphora solaniThecaphora solanit0
- Treatment
- wateragar mediume1
- Outcome
- very slow radial-growing, hyaline, and septate myceliume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Thecaphora solani”
e1“A very slow radial-growing, hyaline, and septate mycelium, as usually seen in most of the in vitro-cultivated filamentous fungi, was obtained in wateragar medium after 30 to 40 days”
Claim · model-written summary
A white, sponge-like mycelial mass was obtained in HCM + 1% activated charcoal and on potato dextrose agar or malt-yeast-peptone media after 40 to 50 days under lab conditions.
- Taxon
- Thecaphora solaniThecaphora solanit0
- Outcome
- white, sponge-like mycelial masse1
- Setting
- lab conditionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Thecaphora solani”
e1“a white, sponge-like mycelial mass was obtained in HCM + 1% activated charcoal, and on common potato dextrose agar or malt-yeast-peptone solid or liquid media, after 40 to 50 days under lab conditions”
Claim · model-written summary
The identity of teliospores and the sponge-like mycelial mass was corroborated by DNA fingerprinting and partial LSU rDNA sequencing.
- Taxon
- Thecaphora solaniThecaphora solanit0
- Outcome
- identity among teliospores and the sponge-like mycelial masse1
- Qualifier
- corroborated by DNA fingerprinting and partial sequencing of the large subunit (LSU) rDNA regione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Thecaphora solani”
e1“The identity among teliospores and the sponge-like mycelial mass was corroborated by DNA fingerprinting and partial sequencing of the large subunit (LSU) rDNA region”
Claim · model-written summary
The sexual cycle of the pathogen was completed under lab conditions, based on teliospore development on the sponge-like mycelial mass.
- Taxon
- Thecaphora solaniThecaphora solanit0
- Outcome
- sexual cycle of the pathogen was completede1
- Setting
- lab conditionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Thecaphora solani”
e1“The sexual cycle of the pathogen was completed under lab conditions based on the development of teliospores on the sponge-like mycelial mass”
Claim · model-written summary
Inoculation of an in vitro cv. Desirée potato explant with radial-growing mycelia induced a gall.
- Taxon
- potato
Solanum tuberosume1 - Material
- cv. Desiréee1
- Organ / stage
- explante1
- Treatment
- inoculated with radial-growing myceliae1
- Interactor
- Thecaphora solaniThecaphora solanit0pathogen
- Outcome
- galle1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Thecaphora solani”
e1“inducing a gall in a cv. Desirée potato explant cultivated in vitro inoculated with radial-growing mycelia”
Claim · model-written summary
Phylogenetic analysis of LSU rDNA data of Thecaphora and other smut fungi confirmed the classification of the pathogen as T. solani.
- Taxon
- T. solaniThecaphora solanie1
- Outcome
- initial classification of the pathogen as T. solanie1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Phylogenetic analysis of LSU rDNA data of the genus Thecaphora and other smut fungi confirmed the initial classification of the pathogen as T. solani”