Abstract
Molecular cytogenetics, particularly the localization of DNA sequences by in situ hybridization, has increased our understanding about the genomic structure of plants and animals. We demonstrate here the application of an improved nonfluorescent in situ hybridization system detection (DAKO GenPoint system) to plant chromosomes. Using this system, highly repetitive 18S-25S rRNA genes were mapped on Vicia faba chromosomes (2n = 12). The modified method of this horseradish peroxidase based enzymatic detection system gave satisfactory results that are comparable to fluorescent signal detection.
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Claims 2
Claim · model-written summary
The nonfluorescent system mapped 18S-25S rRNA genes on Vicia faba chromosomes.
- Taxon
- Vicia faba
Vicia fabae0 - Organ / stage
- chromosomese0
- Gene
- 18S-25S rRNA genese0
- Outcome
- 18S-25S rRNA genes were mappede0
- Value
- 2n = 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using this system, highly repetitive 18S-25S rRNA genes were mapped on Vicia faba chromosomes (2n = 12).”
Claim · model-written summary
The modified enzymatic detection method gave satisfactory results comparable to fluorescent detection.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- satisfactory resultse0
- Direction
- comparablee0
- Comparator
- fluorescent signal detectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The modified method of this horseradish peroxidase based enzymatic detection system gave satisfactory results that are comparable to fluorescent signal detection.”
e1“Vicia faba chromosomes (2n = 12)”