Abstract
This study demonstrates a protocol of polyploidy induction in Salvia hains, in order obtained tetraploid five concentrations (0.1, 0.3, 0.4, 0.5 and 0.7%) of colchicine were used to determine the best treatment for the induction of tetraploid plants. Treatment seeds with colchicine for 24 h product tetraploid plants. Ploidy level of the resulting plantlets was determined by morphological study, chromosome counting and flow cytometry. Tetraploid plants were obtained from S. hains after applied concentrations 0.3, 0.4 and 0.5% of colchicine. Plants obtained from 0.1% colchicine were all diploids. The induced tetraploid in S. hains was accompanied by larger stomata increase in chloroplast number in guard cells and decrease in stomata density, compared to diploid (control) plants. The techniques used to induce tetraploid S. hains plants by colchicine were successful and could be used in breeding programs. Key words: Autotetrapolid, flowcytometry, polyploid, Salvia hains, chromosome counting.
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Claims 7
Claim · model-written summary
Tetraploid Salvia hains plants were obtained after applying 0.3, 0.4 and 0.5% colchicine.
Not reportedComparatorDirection
Verbatim
t0“Salvia hains”
e1“Tetraploid plants were obtained from S. hains after applied concentrations 0.3, 0.4 and 0.5% of colchicine”
Claim · model-written summary
Plants obtained from 0.1% colchicine were all diploid.
Not reportedComparatorDirection
Verbatim
t0“Salvia hains”
e2“Plants obtained from 0.1% colchicine were all diploids”
e3“Ploidy level of the resulting plantlets was determined by morphological study, chromosome counting and flow cytometry”
Claim · model-written summary
Colchicine-induced tetraploid S. hains plants had larger stomata than diploid control plants.
Verbatim
t0“Salvia hains”
e4“The induced tetraploid in S. hains was accompanied by larger stomata”
e5“compared to diploid (control) plants”
Claim · model-written summary
Colchicine-induced tetraploid S. hains plants had an increase in chloroplast number in guard cells relative to diploid control plants.
Verbatim
t0“Salvia hains”
e4“The induced tetraploid in S. hains was accompanied by larger stomata”
e5“compared to diploid (control) plants”
e6“increase in chloroplast number in guard cells”
Claim · model-written summary
Colchicine-induced tetraploid S. hains plants had a decrease in stomata density relative to diploid control plants.
Verbatim
t0“Salvia hains”
e4“The induced tetraploid in S. hains was accompanied by larger stomata”
e5“compared to diploid (control) plants”
e7“decrease in stomata density”
Claim · model-written summary
The colchicine technique for tetraploid S. hains could be used in breeding programs.
Verbatim
t0“Salvia hains”
e7“could be used in breeding programs”
Claim · model-written summary
The techniques used to induce tetraploid S. hains by colchicine were successful.
Verbatim
t0“Salvia hains”
e6“The techniques used to induce tetraploid S. hains plants by colchicine were successful”