Abstract
AbstractMonobromobenzene and monobromonaphthalene proved easier to handle than paradichlorobenzene and acenaphthene, and at least as effective for inhibiting spindle formation, for shortening and straightening chromosomes to permit accurate counts and size comparisons. For prophase studies, methyl alcohol pretreatment was found effective in revealing centromeres, heterochromatin, and knobs. The following schedules were found simple and effective.For metaphase chromosomes: (1) Remove root tip from germinating seed and place in saturated aqueous monobromonaphthalene for three hours. (2) Pour solution from vial and replace with a mixture of 70 volumes 95% ethanol and 30 volumes glacial acetic acid. Leave in fixative at least two days. (3) Remove opaque tip from root and macerate in a drop of acetic-orcein. Cover, heat to near boiling, flatten by pressing slide, cover down, onto a thick blotter.For prophase chromosomes: (1) Remove root tip from germinating seed and place in 3% aqueous methanol for three hours. (2) Pour solution from vial and replace with a mixture of 65 volumes methanol, 5 of chloroform, and 30 of glacial acetic acid. Leave in fixative at least two days. (3) Remove opaque tip from root and macerate in a drop of acetic lacmoid. Cover, heat to near boiling, flatten by pressing slide, cover down, onto a thick blotter.
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Claims 3
Claim · model-written summary
Monobromobenzene and monobromonaphthalene were easier to handle than paradichlorobenzene and acenaphthene.
Verbatim
e0“Monobromobenzene and monobromonaphthalene proved easier to handle than paradichlorobenzene and acenaphthene, and at least as effective for inhibiting spindle formation, for shortening and straightening chromosomes to permit accurate counts and size comparisons”
Claim · model-written summary
Monobromobenzene and monobromonaphthalene were at least as effective as paradichlorobenzene and acenaphthene for inhibiting spindle formation and shortening chromosomes.
Verbatim
e0“Monobromobenzene and monobromonaphthalene proved easier to handle than paradichlorobenzene and acenaphthene, and at least as effective for inhibiting spindle formation, for shortening and straightening chromosomes to permit accurate counts and size comparisons”
Claim · model-written summary
Methyl alcohol pretreatment was effective in revealing centromeres, heterochromatin and knobs in prophase chromosomes.
- Treatment
- methyl alcohol pretreatmente1
- Outcome
- revealing centromeres, heterochromatin, and knobse1
- Direction
- effectivee1
Not reportedTaxonComparator
Verbatim
e1“For prophase studies, methyl alcohol pretreatment was found effective in revealing centromeres, heterochromatin, and knobs”