Abstract
We have confirmed that two closely related circular RNA molecules previously named grapevine yellow speckel viroid (GYSV) and grapevine viroid 1B (GV1B) are indeed viroids. Electron microscopy after spreading under non-denaturing conditions revealed that GYSV has a rod-like structure typical of viroids. Purified GYSV and GV1B replicated independently in inoculated grapevine seedlings and some of the infected plants developed yellow speckle symptoms indicating that both viroids can cause grapevine yellow speckle disease. Plus-sense RNA transcripts derived from a dimeric GYSV cDNA clone induced yellow speckle symptoms in a grapevine seedling confirming the role of GYSV in the yellow speckle disease. Two oligonucleotide probes were synthesized for the detection of the two related viroids. The probes which could detect each viroid individually were used to assess correlations between the occurrence of these viroids and the incidence of the disease.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Two closely related circular RNA molecules, GYSV and GV1B, were confirmed as viroids.
- Interactor
- GYSVPhyllomonavirus gysingensee0pathogen
- Second interactor
- GV1Be0pathogen
- Outcome
- are indeed viroidse0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have confirmed that two closely related circular RNA molecules previously named grapevine yellow speckel viroid (GYSV) and grapevine viroid 1B (GV1B) are indeed viroids”
Claim · model-written summary
Electron microscopy showed that GYSV has a rod-like structure typical of viroids.
- Interactor
- GYSVPhyllomonavirus gysingensee1pathogen
- Outcome
- rod-like structuree1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Electron microscopy after spreading under non-denaturing conditions revealed that GYSV has a rod-like structure typical of viroids”
e0“We have confirmed that two closely related circular RNA molecules previously named grapevine yellow speckel viroid (GYSV) and grapevine viroid 1B (GV1B) are indeed viroids”
Claim · model-written summary
Purified GYSV and GV1B replicated independently in inoculated grapevine seedlings.
- Interactor
- GYSVPhyllomonavirus gysingensee2pathogen
- Second interactor
- GV1Be2pathogen
- Outcome
- replicated independentlye2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Purified GYSV and GV1B replicated independently in inoculated grapevine seedlings and some of the infected plants developed yellow speckle symptoms indicating that both viroids can cause grapevine yellow speckle disease”
Claim · model-written summary
Inoculated grapevine seedlings, some of them infected, developed yellow speckle symptoms after GYSV or GV1B inoculation.
- Treatment
- inoculatede2
- Interactor
- GYSVPhyllomonavirus gysingensee2pathogen
- Second interactor
- GV1Be2pathogen
- Outcome
- yellow speckle symptomse2
- Qualifier
- some of the infected plantse2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Purified GYSV and GV1B replicated independently in inoculated grapevine seedlings and some of the infected plants developed yellow speckle symptoms indicating that both viroids can cause grapevine yellow speckle disease”
Claim · model-written summary
Plus-sense RNA transcripts from a dimeric GYSV cDNA clone induced yellow speckle symptoms in a grapevine seedling.
- Treatment
- Plus-sense RNA transcripts derived from a dimeric GYSV cDNA clonee3
- Interactor
- GYSVPhyllomonavirus gysingensee3pathogen
- Outcome
- yellow speckle symptomse3
- Direction
- inducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Plus-sense RNA transcripts derived from a dimeric GYSV cDNA clone induced yellow speckle symptoms in a grapevine seedling confirming the role of GYSV in the yellow speckle disease”
Claim · model-written summary
The two oligonucleotide probes could detect each of the two related viroids individually.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Two oligonucleotide probes were synthesized for the detection of the two related viroids”
e5“The probes which could detect each viroid individually”
e0“grapevine yellow speckel viroid (GYSV) and grapevine viroid 1B (GV1B)”