Abstract
When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins, its lipopolysaccharide (LPS) sugar composition was changed in two ways: greatly decreased content of what is normally the terminal residue of the LPS, di-O-methylfucose, and a doubling of the 2-O-methylation of other fucose residues in the LPS O antigen. R. etli strain CE395 was isolated after Tn5 mutagenesis of strain CE3 by screening for mutant colonies that did not change antigenically in the presence of seed extract. The LPS of this strain completely lacked 2-O-methylfucose, regardless of whether anthocyanins were present during growth. The mutant gave only pseudonodules in association with P. vulgaris. Interpretation of this phenotype was complicated by a second LPS defect exhibited by the mutant: its LPS population had only about 50% of the normal amount of O-antigen-containing LPS (LPS I). The latter defect could be suppressed genetically such that the resulting strain (CE395 alpha 395) synthesized the normal amount of an LPS I that still lacked 2-O-methylfucose residues. Strain CE395 alpha 395 did not elicit pseudonodules but resulted in significantly slower nodule development, fewer nodules, and less nitrogenase activity than lps(+) strains. The relative symbiotic deficiency was more severe when seeds were planted and inoculated with bacteria before they germinated. These results support previous conclusions that the relative amount of LPS I on the bacterial surface is crucial in symbiosis, but LPS structural features, such as 2-O-methylation of fucose, also may facilitate symbiotic interactions.
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Claims 11
Claim · model-written summary
Growth with bean seed extracts containing anthocyanins greatly decreased the content of di-O-methylfucose, the normal terminal residue of the rhizobial LPS.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise0 - Treatment
- seed extracts containing anthocyaninse0
- Interactor
- Rhizobium etli CE3Rhizobium etlie0symbiont
- Outcome
- terminal residue of the LPS, di-O-methylfucosee1
- Direction
- greatly decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins”
e1“greatly decreased content of what is normally the terminal residue of the LPS, di-O-methylfucose”
Claim · model-written summary
Growth with bean seed extracts doubled the 2-O-methylation of other fucose residues in the rhizobial LPS O antigen.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- seed extracts containing anthocyaninse0
- Interactor
- Rhizobium etli CE3Rhizobium etlie0symbiont
- Outcome
- 2-O-methylation of other fucose residues in the LPS O antigene2
- Direction
- doublinge2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins”
e2“a doubling of the 2-O-methylation of other fucose residues in the LPS O antigen”
Claim · model-written summary
The mutant strain CE395 LPS completely lacked 2-O-methylfucose whether or not anthocyanins were present during growth.
- Taxon
- P. vulgarisPhaseolus vulgarise4
- Outcome
- 2-O-methylfucosee3
- Qualifier
- regardless of whether anthocyanins were present during growthe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The LPS of this strain completely lacked 2-O-methylfucose, regardless of whether anthocyanins were present during growth”
e4“The mutant gave only pseudonodules in association with P. vulgaris”
Claim · model-written summary
The mutant gave only pseudonodules on P. vulgaris.
- Taxon
- P. vulgarisPhaseolus vulgarise4
- Outcome
- only pseudonodulese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The mutant gave only pseudonodules in association with P. vulgaris”
Claim · model-written summary
Strain CE395 alpha 395 had significantly slower nodule development than lps(+) strains.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- CE395 alpha 395e5
- Interactor
- CE395 alpha 395e5symbiont
- Outcome
- nodule developmente5
- Direction
- slowere5
- Comparator
- lps(+) strainse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins”
e5“Strain CE395 alpha 395 did not elicit pseudonodules but resulted in significantly slower nodule development, fewer nodules, and less nitrogenase activity than lps(+) strains”
Claim · model-written summary
Strain CE395 alpha 395 formed fewer nodules than lps(+) strains.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- CE395 alpha 395e5
- Interactor
- CE395 alpha 395e5symbiont
- Outcome
- nodulese5
- Direction
- fewere5
- Comparator
- lps(+) strainse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins”
e5“Strain CE395 alpha 395 did not elicit pseudonodules but resulted in significantly slower nodule development, fewer nodules, and less nitrogenase activity than lps(+) strains”
Claim · model-written summary
Strain CE395 alpha 395 had less nitrogenase activity than lps(+) strains.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- CE395 alpha 395e5
- Interactor
- CE395 alpha 395e5symbiont
- Outcome
- nitrogenase activitye5
- Direction
- lesse5
- Comparator
- lps(+) strainse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins”
e5“Strain CE395 alpha 395 did not elicit pseudonodules but resulted in significantly slower nodule development, fewer nodules, and less nitrogenase activity than lps(+) strains”
Claim · model-written summary
Strain CE395 alpha 395 did not elicit pseudonodules.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Outcome
- pseudonodulese5
- Qualifier
- did not elicite5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins”
e5“Strain CE395 alpha 395 did not elicit pseudonodules but resulted in significantly slower nodule development, fewer nodules, and less nitrogenase activity than lps(+) strains”
Claim · model-written summary
Planting and inoculating seeds with bacteria before germination made the symbiotic deficiency more severe.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- planted and inoculated with bacteria before they germinatede6
- Outcome
- relative symbiotic deficiencye6
- Direction
- more severee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins”
e6“The relative symbiotic deficiency was more severe when seeds were planted and inoculated with bacteria before they germinated”
Claim · model-written summary
The relative amount of LPS I on the bacterial surface appears crucial for symbiosis.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Outcome
- symbiosise7
- Qualifier
- the relative amount of LPS I on the bacterial surface is cruciale7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins”
e7“the relative amount of LPS I on the bacterial surface is crucial in symbiosis”
Claim · model-written summary
LPS structural features such as 2-O-methylation of fucose may facilitate symbiotic interactions.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Outcome
- symbiotic interactionse1
- Qualifier
- 2-O-methylation of fucosee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins”
e1“LPS structural features, such as 2-O-methylation of fucose, also may facilitate symbiotic interactions”
The same questions in other papers
inoculation · Bacteria · Phaseolus vulgaris (common bean)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.