Abstract
SUMMARY: A non-helical strain of Spiroplasma citri from little-leaf diseased oranges was subcultured over 40 times in different media without producing helical cells. It was non-motile and produced colonies of a characteristic morphology on soft agar plates. Serology, DNA hybridization and toxin production confirmed that it was a strain of S. citri but its membrane protein pattern after polyacrylamide gel electrophoresis differed from those of other S. citri strains in that one band was absent. The organism caused symptoms in plants identical to those produced by a pathogenic helical strain of S. citri.
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Claims 5
Claim · model-written summary
A non-helical S. citri strain from little-leaf diseased oranges did not produce helical cells after over 40 subcultures in different media.
- Taxon
- Spiroplasma citriSpiroplasma citrie0
- Outcome
- without producing helical cellse0
- Setting
- different mediae0
Verbatim
e0“A non-helical strain of Spiroplasma citri from little-leaf diseased oranges was subcultured over 40 times in different media without producing helical cells”
Claim · model-written summary
The non-helical S. citri strain was non-motile and produced colonies of characteristic morphology on soft agar plates.
- Taxon
- Spiroplasma citriSpiroplasma citrit0
- Outcome
- non-motile and produced colonies of a characteristic morphologye0
- Setting
- soft agar platese0
Verbatim
t0“Spiroplasma citri”
e0“It was non-motile and produced colonies of a characteristic morphology on soft agar plates”
Claim · model-written summary
Serology, DNA hybridization and toxin production confirmed that the non-helical isolate was a strain of S. citri.
- Taxon
- Spiroplasma citriSpiroplasma citrit0
- Outcome
- confirmed that it was a strain of S. citrie0
- Qualifier
- Serology, DNA hybridization and toxin productione0
Verbatim
t0“Spiroplasma citri”
e0“Serology, DNA hybridization and toxin production confirmed that it was a strain of S. citri”
Claim · model-written summary
The membrane protein pattern of the non-helical strain differed from other S. citri strains in that one band was absent.
- Taxon
- Spiroplasma citriSpiroplasma citrit0
- Outcome
- membrane protein patterne0
- Qualifier
- one band was absente0
- Comparator
- other S. citri strainse0
Not reportedDirection
Verbatim
t0“Spiroplasma citri”
e0“its membrane protein pattern after polyacrylamide gel electrophoresis differed from those of other S. citri strains in that one band was absent”
Claim · model-written summary
The non-helical strain caused symptoms in plants identical to those produced by a pathogenic helical S. citri strain.
- Taxon
- plantsViridiplantaee0
- Interactor
- S. citriSpiroplasma citrie0pathogen
- Outcome
- symptomse0
- Comparator
- pathogenic helical strain of S. citrie0
Not reportedDirection
Verbatim
e0“The organism caused symptoms in plants identical to those produced by a pathogenic helical strain of S. citri”