Abstract
Rice transcription factor RF2a binds to the BoxII cis element of the promoter of rice tungro bacilliform virus and activates promoter expression. The acidic acid-rich domain of RF2a is a transcription activator and has been partially characterized (Dai et al., 2003). The RF2a acidic domain (A; amino acids 49-116) was fused with the synthetic zinc finger ZF-TF 2C7 and was co-introduced with a reporter gene into transgenic Arabidopsis plants. Expression of the reporter gene was increased up to seven times by the effector. In transient assays in tobacco BY-2 protoplasts, we identified a subdomain comprising amino acids 56-84 (A5) that was equally as effective as an activator as the entire acidic domain. A chemically inducible system was used to show determined that A and A5 domains are equally as effective in transcription activation as the well-characterized VP16 activation domain. Bioinformatics analyses revealed that the A5 domain is present only in b-ZIP transcription factors. In dicots, the A domain contains an insertion of four amino acids that is not present in monocot proteins. The A5 domain, and similar domains in other b-ZIP transcription factors, is predicted to form an anti-parallel beta sheet structure.
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Claims 6
Claim · model-written summary
The RF2a acidic domain fused to ZF-TF 2C7 increased reporter expression up to seven times in transgenic Arabidopsis
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- fused with the synthetic zinc finger ZF-TF 2C7e1
- Gene
- RF2ae1
- Outcome
- Expression of the reporter genee2
- Direction
- increasede2
- Value
- up to seven timese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The RF2a acidic domain (A; amino acids 49-116) was fused with the synthetic zinc finger ZF-TF 2C7 and was co-introduced with a reporter gene into transgenic Arabidopsis plants”
e2“Expression of the reporter gene was increased up to seven times by the effector”
Claim · model-written summary
The A5 subdomain (amino acids 56-84) was as effective an activator as the entire acidic domain in tobacco BY-2 protoplasts
Verbatim
e3“In transient assays in tobacco BY-2 protoplasts, we identified a subdomain comprising amino acids 56-84 (A5) that was equally as effective as an activator as the entire acidic domain”
Claim · model-written summary
The A and A5 domains were as effective in transcription activation as the VP16 activation domain
- Taxon
- rice
Oryza sativat0 - Treatment
- A and A5 domainse4
- Outcome
- transcription activatione4
- Direction
- equally as effectivee4
- Comparator
- VP16 activation domaine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“A and A5 domains are equally as effective in transcription activation as the well-characterized VP16 activation domain”
t0“rice transcription factor”
Claim · model-written summary
The A5 domain is present only in b-ZIP transcription factors
- Taxon
- riceOryza sativat0
- Gene
- A5 domaine5
- Outcome
- present only in b-ZIP transcription factorse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Bioinformatics analyses revealed that the A5 domain is present only in b-ZIP transcription factors”
t0“rice transcription factor”
Claim · model-written summary
The A domain contains a four-amino-acid insertion in dicots that is absent from monocot proteins
Verbatim
e6“In dicots, the A domain contains an insertion of four amino acids that is not present in monocot proteins”
Claim · model-written summary
The A5 domain is predicted to form an anti-parallel beta sheet structure
- Taxon
- riceOryza sativat0
- Gene
- A5 domaine7
- Outcome
- form an anti-parallel beta sheet structuree7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“The A5 domain, and similar domains in other b-ZIP transcription factors, is predicted to form an anti-parallel beta sheet structure”
t0“rice transcription factor”