Abstract
Inter-simple sequence repeat (ISSR) markers were used to evaluate genetic divergence among eight Coffea species and to identify the parentage of six interspecific hybrids. A total of 14 primers which contained different simple sequence repeats (SSR) were used as single primers or combined in pairs and tested for PCR amplifications. Two hundred and thirty highly reproducible fragments were amplified, which were then used to estimate the genetic similarity and to cluster the Coffea species and hybrids. High levels of interspecific genetic variation were revealed. The dinucleotide motif (GA)9T combined with other di- tri- and tetra-nucleotides produced a greater number of DNA fragments, mostly polymorphics, suggesting a high frequency of the poly GA microsatellite motifs in the Coffea genomes. The genetic similarity ranged from 0.25 between C. racemosa and C. liberica var. dewevrei to 0.86 between C. arabica var. arabica and Hybrid N. 2. The C. arabica species shared most of its markers with five of the six hybrids suggesting that it is the most likely candidate as one of the progenitors of those hybrids. These results revealed that ISSR markers could be efficiently used for genetic differentiation of the Coffea species and to identify the parentage of Coffea interspecific hybrids.
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Claims 4
Claim · model-written summary
ISSR markers revealed high interspecific genetic variation in Coffea.
- Outcome
- High levels of interspecific genetic variatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High levels of interspecific genetic variation were revealed.”
t0“Coffea species”
Claim · model-written summary
Coffea genetic similarity ranged from 0.25 to 0.86 among the named pairs.
- Outcome
- genetic similaritye0
- Value
- 0.25e0
- Qualifier
- 0.86 between C. arabica var. arabica and Hybrid N. 2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genetic similarity ranged from 0.25 between C. racemosa and C. liberica var. dewevrei to 0.86 between C. arabica var. arabica and Hybrid N. 2.”
t0“Coffea species”
Claim · model-written summary
C. arabica shared most markers with five of six hybrids and was inferred to be a likely parent.
- Taxon
- C. arabica
Coffea arabicae0 - Outcome
- shared most of its markers with five of the six hybridse0
- Value
- five of the six hybridse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The C. arabica species shared most of its markers with five of the six hybrids suggesting that it is the most likely candidate as one of the progenitors of those hybrids.”
Claim · model-written summary
ISSR markers efficiently differentiated Coffea species and identified interspecific hybrid parentage.
- Outcome
- genetic differentiation of the Coffea species and to identify the parentage of Coffea interspecific hybridse0
- Value
- ISSR markerse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ISSR markers could be efficiently used for genetic differentiation of the Coffea species and to identify the parentage of Coffea interspecific hybrids.”