Abstract
Fifty-eight new isolates were obtained from root nodules of common bean (Phaseolus vulgaris) cultivated in soils originating from different agroecological areas in Senegal and Gambia (West Africa). A polyphasic approach including both phenotypic and genotypic techniques was used to study the diversity of the 58 Rhizobium isolates and to determine their taxonomic relationships with reference strains. All the techniques performed, analysis of multilocus enzyme electrophoretic patterns, SDS-PAGE profiles of total cell proteins, PCR-RFLP analysis of the genes encoding 16S rRNA and of the 16S-23S RNA intergenic spacer region (ITS-PCR-RFLP), auxanographic tests using API galleries and nodulation tests lead to the consensus conclusion that the new rhizobial isolates formed two main distinct groups, I and II, belonging to Rhizobium tropici type B and Rhizobium etli, respectively. By MLEE R. etli and group II strains showed several related electrophoretic types, evidencing some extent of internal heterogeneity among them. This heterogeneity was confirmed by other techniques (ITS-PCR-RFLP, SDS-PAGE and host-plant-specificity) with the same nine distinct strains of group II showing some differences from the core of group II (54 strains).
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Claims 3
Claim · model-written summary
Bean-nodulating isolates formed two distinct groups corresponding to Rhizobium tropici type B and Rhizobium etli.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise1 - Interactor
- Rhizobium tropici type B
Martinezella tropicie0symbiont - Outcome
- two main distinct groups, I and IIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“new rhizobial isolates formed two main distinct groups, I and II, belonging to Rhizobium tropici type B and Rhizobium etli, respectively”
e1“common bean (Phaseolus vulgaris)”
Claim · model-written summary
Group II and Rhizobium etli strains had multiple related electrophoretic types, indicating internal heterogeneity.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise1
- Interactor
- R. etliRhizobium etlie0symbiont
- Outcome
- several related electrophoretic typese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“R. etli and group II strains showed several related electrophoretic types, evidencing some extent of internal heterogeneity among them”
e1“common bean (Phaseolus vulgaris)”
Claim · model-written summary
Nine group II strains differed from the group II core in molecular and host specificity tests.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise1
- Outcome
- some differences from the core of group IIe0
- Value
- nine distinct strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the same nine distinct strains of group II showing some differences from the core of group II (54 strains)”
e1“common bean (Phaseolus vulgaris)”