Abstract
A 6·7‐kb DNA fragment has been isolated from an R‐type isolate of Pseudocercosporella herpotrichoides that exhibits specific hybridization to R‐type isolates and not to W, C or S pathotypes or to P. anguioides. R‐type isolates are polymorphic with respect to this fragment and three sizes were present (11·6, 6·7 and 4·5 kb) in Eco RI‐digested DNA from isolates of a world‐wide collection. Infection of rye seedlings was demonstrated by hybridization of this probe to DNA extracted from infected plants.
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Claims 3
Claim · model-written summary
The DNA fragment hybridized specifically to R-type isolates rather than other pathotypes.
- Interactor
- Pseudocercosporella herpotrichoides
Oculimacula yallundaee0pathogen - Outcome
- specific hybridization to R‐type isolatese0
- Value
- 6·7‐kbe0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 6·7‐kb DNA fragment has been isolated from an R‐type isolate of Pseudocercosporella herpotrichoides that exhibits specific hybridization to R‐type isolates and not to W, C or S pathotypes or to P. anguioides”
Claim · model-written summary
R-type isolates carried three fragment sizes.
Not reportedTaxon
Verbatim
e0“R‐type isolates are polymorphic with respect to this fragment and three sizes were present (11·6, 6·7 and 4·5 kb) in Eco RI‐digested DNA from isolates of a world‐wide collection”
Claim · model-written summary
Probe hybridization demonstrated infection of rye seedlings.
- Taxon
- rye
Secale cerealee0 - Outcome
- Infection of rye seedlingse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection of rye seedlings was demonstrated by hybridization of this probe to DNA extracted from infected plants”