Abstract
Virus-induced gene silencing (VIGS) is a powerful tool for the study of gene function in plants. Here we report that either by syringe-infiltrating the tobacco rattle virus (TRV)-vector into the surface, stem or carpopodium of a tomato fruit attached to the plant or by vacuum-infiltrating into a tomato fruit detached from the plant, TRV can efficiently spread and replicate in the tomato fruit. Although VIGS can be performed in tomato fruit by all of the means mentioned above, the most effective method is to inject the TRV-vector into the carpopodium of young fruit attached to the plant about 10 days after pollination. Several reporter genes related to ethylene responses and fruit ripening, including LeCTR1 and LeEILs genes, were also successfully silenced by this method during fruit development. In addition, we found that the silencing of the LeEIN2 gene results in the suppression of tomato fruit ripening. The results of our study indicate that the application of VIGS techniques by the described methods can be successfully applied to tomato fruit and is a valuable tool for studying functions of the relevant genes during fruit developing.
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Claims 5
Claim · model-written summary
TRV spread and replicated efficiently in tomato fruit after syringe or vacuum infiltration.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- fruite0
- Treatment
- tobacco rattle virus (TRV)-vectore0
- Interactor
- tobacco rattle virusTobacco rattle viruse0pathogen
- Outcome
- TRV can efficiently spread and replicate in the tomato fruite0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“either by syringe-infiltrating the tobacco rattle virus (TRV)-vector into the surface, stem or carpopodium of a tomato fruit attached to the plant or by vacuum-infiltrating into a tomato fruit detached from the plant, TRV can efficiently spread and replicate in the tomato fruit”
Claim · model-written summary
Carpopodium injection of young attached tomato fruit around 10 days after pollination was the most effective VIGS method.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- young fruite0
- Treatment
- inject the TRV-vector into the carpopodiume0
- Outcome
- the most effective methode0
- Qualifier
- about 10 days after pollinatione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the most effective method is to inject the TRV-vector into the carpopodium of young fruit attached to the plant about 10 days after pollination”
t0“tomato fruit”
Claim · model-written summary
LeCTR1 was successfully silenced during fruit development.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- fruite0
- Gene
- LeCTR1e0
- Outcome
- successfully silencede0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“including LeCTR1 and LeEILs genes, were also successfully silenced by this method during fruit development”
t0“tomato fruit”
Claim · model-written summary
LeEILs genes were successfully silenced during fruit development.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- fruite0
- Gene
- LeEILse0
- Outcome
- successfully silencede0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LeCTR1 and LeEILs genes, were also successfully silenced by this method during fruit development”
t0“tomato fruit”
Claim · model-written summary
Silencing LeEIN2 suppressed tomato fruit ripening.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- silencing of the LeEIN2 genee0
- Gene
- LeEIN2e0
- Outcome
- tomato fruit ripeninge0
- Direction
- suppressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of the LeEIN2 gene results in the suppression of tomato fruit ripening”
The same questions in other papers
loss of function · quality: postharvest · Solanum lycopersicum (tomato)Positive and negative results
32 papers in the same direction as this paper · 21 in the opposite direction
- positive
- positive
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See all 54 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.