Abstract
The non-structural protein Pns12 of Rice dwarf virus is one of the early proteins expressed in cultured insect cells, and it is one of 12 proteins that initiate the formation of the viroplasm, the putative site of viral replication. Pns4 is also a non-structural protein, visible as minitubules after nucleation of the viroplasm. We introduced Pns12- and Pns4-specific RNA interference (RNAi) constructs into rice plants. The resultant transgenic plants accumulated short interfering RNAs specific to the constructs. The progeny of rice plants with Pns12-specific RNAi constructs, after self-fertilization, were strongly resistant to viral infection. By contrast, resistance was less apparent in the case of rice plants with Pns4-specific RNAi constructs, and delayed symptoms appeared in some plants of each line. Our results suggest that interference with the expression of a protein that is critical for viral replication, such as the viroplasm matrix protein Pns12, might be a practical and effective way to control viral infection in crop plants.
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Claims 5
Claim · model-written summary
Transgenic rice plants accumulated construct-specific short interfering RNAs.
- Taxon
- rice
Oryza sativat0 - Material
- transgenic plantse0
- Outcome
- accumulated short interfering RNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resultant transgenic plants accumulated short interfering RNAs specific to the constructs.”
t0“rice plants”
Claim · model-written summary
Rice plants carrying Pns12-specific RNAi constructs were strongly resistant to viral infection.
- Taxon
- riceOryza sativae0
- Treatment
- Pns12-specific RNAi constructse0
- Interactor
- virale0pathogen
- Gene
- Pns12e0
- Outcome
- resistant to viral infectione0
- Direction
- strongly resistante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The progeny of rice plants with Pns12-specific RNAi constructs, after self-fertilization, were strongly resistant to viral infection.”
Claim · model-written summary
Resistance was less apparent in rice plants with Pns4-specific RNAi constructs.
- Taxon
- riceOryza sativae0
- Treatment
- Pns4-specific RNAi constructse0
- Interactor
- Rice dwarf virusRice dwarf virust0pathogen
- Gene
- Pns4e0
- Outcome
- resistancee0
- Direction
- less apparente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resistance was less apparent in the case of rice plants with Pns4-specific RNAi constructs”
t0“Rice dwarf virus”
Claim · model-written summary
Delayed symptoms appeared in some plants of each Pns4 RNAi line.
- Taxon
- riceOryza sativat0
- Outcome
- delayed symptoms appearede0
- Qualifier
- some plants of each linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“delayed symptoms appeared in some plants of each line”
t0“rice plants”
Claim · model-written summary
Interference with critical viral replication protein expression may be an effective way to control infection in crop plants.
- Taxon
- riceOryza sativat0
- Gene
- Pns12e0
- Outcome
- control viral infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interference with the expression of a protein that is critical for viral replication, such as the viroplasm matrix protein Pns12, might be a practical and effective way to control viral infection in crop plants”
t0“rice plants”