Abstract
PREMISE OF THE STUDY: The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis. METHODS AND RESULTS: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di- and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus. CONCLUSION: The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species.
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Claims 3
Claim · model-written summary
Nine microsatellite loci were isolated and characterized in I. hypsophila.
- Taxon
- I. hypsophilaIsoetes hypsophilae0
- Outcome
- microsatellite loci were isolated and characterizede0
- Value
- nine microsatellite locie0
Verbatim
e0“nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila”
t0“Isoetes hypsophila”
Claim · model-written summary
The primers amplified repeats with three to 11 alleles per locus.
- Taxon
- Isoetes hypsophilaIsoetes hypsophilat0
- Outcome
- di- and hexanucleotide repeatse0
- Value
- three to 11 alleles per locuse0
Verbatim
e0“primers amplified di- and hexanucleotide repeats with three to 11 alleles per locus”
t0“Isoetes hypsophila”
Claim · model-written summary
Seven of nine primers cross-amplified in I. sinensis with two to seven alleles per locus.
- Taxon
- I. sinensisIsoetes sinensise0
- Outcome
- primers were cross-amplifiede0
- Value
- Seven of nine primerse0
Verbatim
e0“Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus”
t0“Isoetes hypsophila”