Abstract
Citrus tristeza virus (CTV) isolates from 35 grapefruit accessions belonging to Citrus Active Germplasm Bank of the "Instituto Agronômico de Campinas" located at the "Centro APTA Citros Sylvio Moreira", Cordeirópolis, São Paulo state, Brazil, were characterized and evaluated through symptoms in the trees, biological indexing, immunological diagnosis with different monoclonal antibodies and SSCP analysis (single-strand conformation polymorphism) of the coat protein gene. Symptomatology indicated that, in general, the group of plants with smaller canopy volume and severe stem pitting differed significantly from the group that presented greater vegetative development and mild to moderate stem pitting. However, the isolates from most of the accessions induced mild reaction on Mexican lime. The serological evaluation through the DAS-ELISA using monoclonal antibodies did not reveal any association between virus titer in the plant tissue and symptoms. The reaction with different monoclonal antibodies and the distinct electrophoresis patterns obtained through SSCP showed that there is a high degree of diversity among the isolates that infect these grapefruit accessions. High complexity within the same isolate was also observed in the SSCP profiles. This finding indicates that the CTV isolates from these plants are a complex mixture of CTV haplotypes. Similar SSCP banding patterns were observed among some plants with strong stem pitting symptoms, and among some plants with weak or moderate stem pitting symptoms.
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Claims 7
Claim · model-written summary
Grapefruit with smaller canopies and severe stem pitting differed significantly from plants with greater growth and milder pitting.
- Taxon
- grapefruit
_Pompelino_Grapefruit_002.jpg?width=60)
Citrus ×paradisit0 - Interactor
- Citrus tristeza virusCitrus tristeza virust1pathogen
- Outcome
- canopy volumee0
- Direction
- smallere0
- Comparator
- greater vegetative development and mild to moderate stem pittinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Pompelino_Grapefruit_002.jpg?width=500)
Verbatim
t0“grapefruit (Citrus paradisi Macf.)”
t1“Citrus tristeza virus”
e0“the group of plants with smaller canopy volume and severe stem pitting differed significantly from the group that presented greater vegetative development and mild to moderate stem pitting.”
Claim · model-written summary
Most accession isolates produced mild reactions on Mexican lime.
- Taxon
- Mexican limee0
- Interactor
- Citrus tristeza virusCitrus tristeza virust0pathogen
- Outcome
- mild reactione0
Verbatim
e0“the isolates from most of the accessions induced mild reaction on Mexican lime.”
t0“Citrus tristeza virus”
Claim · model-written summary
Virus titer was not associated with grapefruit symptoms.
- Taxon
- grapefruitCitrus ×paradisit0
- Interactor
- Citrus tristeza virusCitrus tristeza virust1pathogen
- Outcome
- virus titer in the plant tissuee0
- Direction
- did not reveal any associatione0
- Comparator
- symptomse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapefruit (Citrus paradisi Macf.)”
t1“Citrus tristeza virus”
e0“did not reveal any association between virus titer in the plant tissue and symptoms.”
Claim · model-written summary
Antibody responses and SSCP patterns showed high diversity among CTV isolates infecting grapefruit.
- Taxon
- grapefruitCitrus ×paradisit0
- Outcome
- high degree of diversity among the isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapefruit (Citrus paradisi Macf.)”
e0“The reaction with different monoclonal antibodies and the distinct electrophoresis patterns obtained through SSCP showed that there is a high degree of diversity among the isolates that infect these grapefruit accessions.”
Claim · model-written summary
SSCP profiles showed high complexity within individual CTV isolates.
- Taxon
- Citrus tristeza virusCitrus tristeza virust0
- Outcome
- High complexity within the same isolatee0
Verbatim
e0“High complexity within the same isolate was also observed in the SSCP profiles.”
t0“Citrus tristeza virus”
Claim · model-written summary
Authors inferred that CTV isolates comprise mixtures of haplotypes.
Verbatim
e0“the CTV isolates from these plants are a complex mixture of CTV haplotypes.”
Claim · model-written summary
Some plants with strong pitting and some with weak or moderate pitting each shared similar SSCP patterns.
- Taxon
- grapefruitCitrus ×paradisit0
- Outcome
- Similar SSCP banding patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapefruit (Citrus paradisi Macf.)”
e0“Similar SSCP banding patterns were observed among some plants with strong stem pitting symptoms, and among some plants with weak or moderate stem pitting symptoms.”