Abstract
Amplified fragment length polymorphism (AFLP) is a PCR-based marker, which is suitable for DNA fingerprinting. The AFLP fingerprinting has not been described in meadowfoam. This powerful method was utilized to access genetic diversity of 41 meadowfoam accessions belonging to the genus Limnanthes. The objectives were to estimate polymorphic information contents (PIC) for AFLP markers and genetic distance among germplasm, and to assess the pattern of genetic diversity in meadowfoam germplasm. One hundred and seventy six polymorphic AFLP markers were produced using 6 primer combinations across 41 accessions. The PIC value ranged from 0.0 to 0.5 and 42 % of germplasm showed high PIC scores in a range between 0.45 and 0.5. Genetic distance ranged from 0.14 to 0.55 with an average of 0.44. The UPGMA clustering phenogram based on the distance matrix was consistent with the known taxonomic classification. The first three principal coordinate analyses accounted for 37 % of total variation of genetic distance estimated. Cluster analysis and principal component analysis clearly separated L. floccosa from L. alba. Within L. alba, subspecies alba and versicolor were distinctly separated into two groups. The results suggested genetic diversity among meadowfoam germplasm was
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Claims 9
Claim · model-written summary
Six primer combinations produced 176 polymorphic AFLP markers across 41 accessions.
Verbatim
e0“One hundred and seventy six polymorphic AFLP markers were produced using 6 primer combinations across 41 accessions”
t0“Meadowfoam”
Claim · model-written summary
AFLP marker PIC ranged from 0.0 to 0.5.
Verbatim
e0“The PIC value ranged from 0.0 to 0.5”
t0“Meadowfoam”
Claim · model-written summary
Forty two percent of germplasm had high PIC scores of 0.45 to 0.5.
Verbatim
e0“42 % of germplasm showed high PIC scores in a range between 0.45 and 0.5”
t0“Meadowfoam”
Claim · model-written summary
Genetic distance ranged from 0.14 to 0.55.
Verbatim
e0“Genetic distance ranged from 0.14 to 0.55 with an average of 0.44”
t0“Meadowfoam”
Claim · model-written summary
Mean genetic distance was 0.44.
Verbatim
e0“Genetic distance ranged from 0.14 to 0.55 with an average of 0.44”
t0“Meadowfoam”
Claim · model-written summary
UPGMA clustering agreed with known taxonomy.
Verbatim
e0“The UPGMA clustering phenogram based on the distance matrix was consistent with the known taxonomic classification”
t0“Meadowfoam”
Claim · model-written summary
The first three principal coordinates accounted for 37% of genetic distance variation.
Verbatim
e0“The first three principal coordinate analyses accounted for 37 % of total variation of genetic distance estimated”
t0“Meadowfoam”
Claim · model-written summary
Cluster and principal component analyses separated L. floccosa from L. alba.
- Taxon
- L. floccosa
Limnanthes floccosae0 - Outcome
- clearly separated L. floccosa from L. albae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cluster analysis and principal component analysis clearly separated L. floccosa from L. alba”
Claim · model-written summary
Within L. alba, subspecies alba and versicolor separated into two groups.
- Taxon
- L. albaLimnanthes albae0
- Material
- subspecies alba and versicolore0
- Outcome
- distinctly separated into two groupse0
Verbatim
e0“Within L. alba, subspecies alba and versicolor were distinctly separated into two groups”