Abstract
Microarray analysis was used to investigate changes in host gene expression during the primary stages of the interaction between the susceptible plant Arabidopsis thaliana (L.) Heynh ecotype Col-0 and the biotrophic pathogen Plasmodiophora brassicae Woronin. Analyses were conducted at 4, 7 and 10 days after inoculation (DAI) and revealed significant induction or suppression of a relatively low number of genes in a range of functional categories. At 4 DAI, there was induced expression of several genes known to be critical for pathogen recognition and signal transduction in other resistant host-pathogen interactions. As the pathogen further colonised root tissue and progressed through the primary plasmodium stage to production of zoosporangia at 7 and 10 DAI, respectively, fewer genes showed changes in expression. The microarray results were validated by examining a subset of induced genes at 4 DAI by quantitative real-time reverse transcriptase PCR (RT-qPCR) analysis all of which correlated positively with the microarray data. The two A. thaliana mutants jar1 and coiI tested were found to be susceptible to P. brassicae. The involvement of defence-related hormones in the interaction was further investigated and the findings indicate that addition of salicylic acid can suppress clubroot disease in A. thaliana plants.
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Claims 6
Claim · model-written summary
Microarray analysis at 4, 7 and 10 days after inoculation revealed significant induction or suppression of a relatively low number of Arabidopsis genes in a range of functional categories.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Interactor
- Plasmodiophora brassicae
.jpg?width=60)
Plasmodiophora brassicaet1pathogen - Outcome
- significant induction or suppression of a relatively low number of genese0
- Qualifier
- at 4, 7 and 10 days after inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Arabidopsis thaliana”
t1“Plasmodiophora brassicae”
e0“Analyses were conducted at 4, 7 and 10 days after inoculation (DAI) and revealed significant induction or suppression of a relatively low number of genes in a range of functional categories”
Claim · model-written summary
At 4 DAI, several genes known to be critical for pathogen recognition and signal transduction were induced in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Interactor
- Plasmodiophora brassicaePlasmodiophora brassicaet1pathogen
- Outcome
- induced expression of several genes known to be critical for pathogen recognition and signal transductione0
- Direction
- inducede0
- Qualifier
- At 4 DAIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
t1“Plasmodiophora brassicae”
e0“At 4 DAI, there was induced expression of several genes known to be critical for pathogen recognition and signal transduction in other resistant host-pathogen interactions”
Claim · model-written summary
At 7 and 10 DAI, as the pathogen colonised root tissue and reached zoosporangia production, fewer genes showed expression changes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- root tissuee0
- Interactor
- Plasmodiophora brassicaePlasmodiophora brassicaet1pathogen
- Outcome
- fewer genes showed changes in expressione0
- Direction
- fewere0
- Qualifier
- at 7 and 10 DAIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
t1“Plasmodiophora brassicae”
e0“As the pathogen further colonised root tissue and progressed through the primary plasmodium stage to production of zoosporangia at 7 and 10 DAI, respectively, fewer genes showed changes in expression”
Claim · model-written summary
RT-qPCR of a subset of genes induced at 4 DAI correlated positively with the microarray data, validating the microarray results.
Verbatim
e0“The microarray results were validated by examining a subset of induced genes at 4 DAI by quantitative real-time reverse transcriptase PCR (RT-qPCR) analysis all of which correlated positively with the microarray data”
Claim · model-written summary
The Arabidopsis mutants jar1 and coiI were susceptible to Plasmodiophora brassicae.
- Taxon
- A. thalianaArabidopsis thalianae0
- Material
- jar1 and coiIe0
- Interactor
- P. brassicaePlasmodiophora brassicaee0pathogen
- Outcome
- susceptiblee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The two A. thaliana mutants jar1 and coiI tested were found to be susceptible to P. brassicae”
Claim · model-written summary
Addition of salicylic acid can suppress clubroot disease in Arabidopsis thaliana plants.
- Taxon
- A. thalianaArabidopsis thalianae0
- Treatment
- addition of salicylic acide0
- Interactor
- Plasmodiophora brassicaePlasmodiophora brassicaet1pathogen
- Outcome
- clubroot diseasee0
- Direction
- suppresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Plasmodiophora brassicae”
e0“addition of salicylic acid can suppress clubroot disease in A. thaliana plants”