Abstract
Isolates of the pathogenic ascomycete Leptosphaeria maculans have been cultured from blackleg-affected oilseed rape (Brassica napus) stubble from Horsham, Victoria. These isolates are indistinguishable on the basis of morphological characters, but can be classified as either aggressive or non-aggressive by their ability to infect B. napus cultivars Midas and Westar. These aggressive and non-aggressive isolates of L. maculans can be distinguished by molecular techniques including electrophoretic karyotyping, Southern analysis of the ribosomal RNA gene repeat, Random Amplified Polymorphic DNA (RAPD) marker analysis, and pigment production. The presence of aggressive and non-aggressive strains of L. maculans in North America and Europe has been previously described. This is the first report of non-aggressive L. maculans strains isolated from B. napus in Australia.
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Claims 3
Claim · model-written summary
Fungal isolates were aggressive or non-aggressive by infection of two rape cultivars.
- Taxon
- B. napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Material
- Midas and Westare0
- Interactor
- Leptosphaeria maculans
Plenodomus lingamt0pathogen - Outcome
- ability to infecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“classified as either aggressive or non-aggressive by their ability to infect B. napus cultivars Midas and Westar”
t0“Leptosphaeria maculans”
Claim · model-written summary
Molecular techniques distinguished aggressive from non-aggressive isolates.
- Taxon
- Brassica napusBrassica napust0
- Interactor
- L. maculansPlenodomus lingame0pathogen
- Outcome
- aggressive and non-aggressive isolates of L. maculanse0
- Value
- distinguishede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These aggressive and non-aggressive isolates of L. maculans can be distinguished by molecular techniques including electrophoretic karyotyping, Southern analysis of the ribosomal RNA gene repeat, Random Amplified Polymorphic DNA (RAPD) marker analysis, and pigment production”
t0“oilseed rape (Brassica napus)”
Claim · model-written summary
Non-aggressive blackleg strains were found on oilseed rape in Australia.
- Taxon
- B. napusBrassica napuse0
- Interactor
- L. maculansPlenodomus lingame0pathogen
- Outcome
- non-aggressive L. maculans strainse0
- Setting
- Australiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This is the first report of non-aggressive L. maculans strains isolated from B. napus in Australia”